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  7. Anti-CD370 PE (Clone : 8F9)

Anti-CD370 PE (Clone : 8F9)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD370 (8F9) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Anti-CD370 PE (Clone : 8F9)
Anti-CD370 PE (Clone : 8F9)
Anti-CD370 PE (Clone : 8F9)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD370 (8F9) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).
Figure 2 : Flow cytometry multicolor surface staining of human peripheral blood mononuclear cells stained using anti-human CD370 (8F9) PE antibody (10 µl reagent / 100 µl of peripheral whole blood) and anti-human HLA-DR (MEM-12) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Figure 3 :Separation of human CD370 positive HLA-DR positive cells (red-filled) from CD370 negative HLA-DR negative cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD370 (8F9) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

Product code: 30-2570

Clone name : 8F9
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : PE

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100 tests
$443.00 

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  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Amount : 100 tests
Isotype : Mouse IgG2a
Purification : The purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Gene : CLEC9A
Gene ID : 283420
Alternative Name : CLEC9A, DNGR1, DNGR-1, UNQ9341,C-type lectin domain containing 9A
Immunogen Information : RBL-2H3 cells expressing human CLEC9A fused to an HA epitope
CD370 / CLEC9A, also known as DNGR1, is a type II transmembrane glycoprotein with extracellular C-type lectin domain and intracellular ITAM-containing domain. Its expression is restricted to BDCA3+ conventional dendritic cells and to a subset of CD14+ CD16- monocytes. CD370 serves as a receptor for ubiquitous preformed acid-labile protein associated ligands that are exposed when the cell membrane is damaged, such as on necrotic cells. Its triggering by these ligands mediates recruitment and activation of the tyrosine kinase Syk and leads to their cross-presentation to the immune system.
Specificity : The mouse monoclonal antibody 8F9 recognizes an extracellular epitope of CD370 / CLEC9A (DNGR1), a type II transmembrane protein functioning as an endocytic receptor on BDCA31+ dendritic cells and on a subset of CD14+ CD16- monocytes.

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-CD370 PE (Clone : 8F9)

Product code: 30-2570
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