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    7. Anti-Human CD16 PE-DyLight® 594 (Clone : 3G8)

    Anti-Human CD16 PE-DyLight® 594 (Clone : 3G8)

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    Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood).

    Anti-Human CD16 PE-DyLight® 594 (Clone : 3G8)
    Anti-Human CD16 PE-DyLight® 594 (Clone : 3G8)
    Anti-Human CD16 PE-DyLight® 594 (Clone : 3G8)

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    Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD16 (3G8) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood).
    Figure 2 : Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD16 (3G8) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood) and anti-human CD3 (UCHT1) Pacific Blue™ antibody (4 µl reagent / 100 µl of peripheral whole blood).
    Figure 3 :Separation of human CD16 positive CD3 negative NK cells (red-filled) from CD16 negative CD3 positive T cells (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD16 (3G8) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood).

    Product code: 30-2645

    Clone name : 3G8
    Clonality : Monoclonal
    Application : FACS
    Reactivity : Human
    Conjugate : PE-DyLight® 594

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    100 tests
    $469.00  $375.20 

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    • Product Info
    • Antigen Details
    • Description
    • Application Note
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    Amount : 100 tests
    Isotype : Mouse IgG1 kappa
    Purification : The purified antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
    Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
    Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
    Gene : FCGR3A
    Gene ID : 2214
    Alternative Name : FcgammaRIII, IGFR3, FCRIII,
    Immunogen Information : Human neutrophils
    CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
    Specificity : The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen). CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils).

    Flow cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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    Anti-Human CD16 PE-DyLight® 594 (Clone : 3G8)

    Product code: 30-2645
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