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  7. Anti-Human CD34 PE-DyLight® 594 (Clone : 4H11[APG])

Anti-Human CD34 PE-DyLight® 594 (Clone : 4H11[APG])

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using anti-human CD34 (4H11[APG]) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood).

Anti-Human CD34 PE-DyLight® 594 (Clone : 4H11[APG])

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Product code: 30-2582

Clone name : 4H11[APG]
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : PE-DyLight® 594

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100 tests
$469.00 

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  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Amount : 100 tests
Isotype : Mouse IgG1
Purification : The purified antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Gene : CD34
Gene ID : 947
Alternative Name : Mucosialin,CD34 molecule
Immunogen Information : Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Specificity : The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34 (Mucosialin), a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class II antibody QBEnd10 and class III antibodies BIRMA K3 and 8G12 on KG1a cell line.

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Human CD34 PE-DyLight® 594 (Clone : 4H11[APG])

Product code: 30-2582
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