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  7. Anti-Human CD5 PE-DyLight<sup>®</sup> 594 (Clone : L17F12)

Anti-Human CD5 PE-DyLight® 594 (Clone : L17F12)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (L17F12) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood).

Anti-Human CD5 PE-DyLight<sup>®</sup> 594 (Clone : L17F12)
Anti-Human CD5 PE-DyLight<sup>®</sup> 594 (Clone : L17F12)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD5 (L17F12) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood).
Figure 2 : Separation of human CD5 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD5 (L17F12) PE-DyLight® 594 antibody (4 µl reagent / 100 µl of peripheral whole blood).

Product code: 30-2555

Clone name : L17F12
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : PE-DyLight® 594

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100 tests
$469.00 

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  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Amount : 100 tests
Isotype : Mouse IgG2a kappa
Purification : The purified antibody is conjugated with tandem dye PE-DyLight® 594 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Gene : CD5
Gene ID : 921
Alternative Name : T1, LEU1,CD5 molecule
Immunogen Information : Human acute lymphoblastic leukemia (ALL) T cells
CD5 antigen (T1; 67 kDa) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains.The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.
Specificity : The mouse monoclonal antibody L17F12 reacts with an extracellular epitope of CD5, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).

Flow cytometry: The reagent is designed for analysis of human blood cells using 4 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Human CD5 PE-DyLight® 594 (Clone : L17F12)

Product code: 30-2555
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