DLK1 Human, HEK
|Alternative Name :||DLK1, Delta-Like 1 Homolog (Drosophila), PREF1, DLK-1, FA1, DLK, PG2, Delta-Like Homolog (Drosophila), Protein Delta Homolog 1, Preadipocyte Factor 1, Fetal Antigen 1, Secredeltin, DELTA1, Pref-1, ZOG.|
|Amount :||10 µg|
Filtered White lyophilized (freeze-dried) powder.
Delta-Like 1, also known as DLK1 is a member of the Notch/Delta/Serrate family of epidermal growth factor (EGF)-like repeat-containing proteins which act as ligands in the Notch signaling pathway. Furthermore, DLK1 has six highly conserved epidermal-growth-factor motifs and it also contains up to ten O-glycosylation and N-glycosylation locations, six of them are differentially glycosylated.
DLK1 Human Recombinant produced in HEK293 Cells is a single, glycosylated, polypeptide chain (a.a 24-303) containing 290 amino acids including a 10 a.a C-terminal His tag. The total molecular mass is 31.2kDa (calculated).
|Purification :||Greater than 95.0% as determined by SDS-PAGE.|
|Content :||DLK1 filtered (0.4 µm) and lyophilized from 0.5mg/ml in PBS and 5% w/v trehalose, pH7.4
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. DLK1 is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
|Storage condition :||Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.|
|Amino acid sequence :||AECFPACNPQ NGFCEDDNVC RCQPGWQGPL CDQCVTSPGC LHGLCGEPGQ CICTDGWDGE LCDRDVRACS SAPCANNRTC VSLDDGLYEC SCAPGYSGKD CQKKDGPCVI NGSPCQHGGT CVDDEGRASH ASCLCPPGFS GNFCEIVANS CTPNPCENDG VCTDIGGDFR CRCPAGFIDK TCSRPVTNCA SSPCQNGGTC LQHTQVSYEC LCKPEFTGLT CVKKRALSPQ QVTRLPSGYG LAYRLTPGVH ELPVQQPEHR ILKVSMKELN KKTPLLTEGQ HHHHHHHHHH.|