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    7. Monoclonal Antibody to human Apoptotic Neutrophils

    Monoclonal Antibody to human Apoptotic Neutrophils

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    Monoclonal Antibody to human Apoptotic Neutrophils

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    Product code: 10-3547

    Clone name : BOB93
    Clonality : Monoclonal
    Application : FACS
    Reactivity : Human

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    Size
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    Available Pack Size(s)

    •   1.0 mg

    •  500 µg

    • $2,100.00 

    • $1,500.00  $1,200.00 

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    • Product Info
    • Antigen Details
    • Description
    • Application Note
    • Review   (0)
    Amount : 500 µg
    Isotype : Mouse IgG1
    Content : 0.5 mg 0.2 µm filtered antibody solution in PBS, containing 0.02% sodium azide and 0.1% bovine serum albumin.
    Storage condition : Product should be stored at 4°C. Under recommended storage conditions, product is stable for one year. cause loss of activity. Under recommended storage conditions, product is stable for one year.

    The monoclonal antibody BOB93 binds to the surface of apoptotic neutrophils. Neutrophils have been implicated in the pathogenenesis of a variety of inflammatory diseases including the adult respiratory distress syndrome, idiopathic pulmonary fibrosis, ulcerative colitis and rheumatoid arthritis. Although the neutrophil is a vital component of the body’s defense against infectious agents, uncontrolled release of its toxic substances may inflict “friendly fire” damage on surrounding tissue and propagate the inflammatory response, leading to scarring and tissue destruction. Apoptosis leads to recognition and safe disposal of dying cells by phagocytosis. Binding of the monoclonal antibody BOB93 to apoptotic neutrophils is dependent on the presence of sialoglycoprotein fetuin, a constituent of bovine serum. Fetuin is the antigen for BOB93, and BOB93 and fetuin form a complex in solution that is necessary and sufficient for binding to apoptotic neutrophils. The antigen recognised by BOB93 may act as a “molecular bridge” between the surface of the apoptotic neutrophil and the phagocyte to modulate apoptotic neutrophil clearance at inflammatory sites. Human macrophage phagocytosis of apoptotic neutrophils was augmented in vitro by addition of the antigen recognised by BOB93, which does not bind to other apoptotic leukocytes.

    For flow cytometry analysis:1:10; Western blotting dilutions: 1:10.

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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    Monoclonal Antibody to human Apoptotic Neutrophils

    Product code: 10-3547
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