Recombinant Mouse Monoclonal Antibody to Histone H1 (Nuclear Marker)(Clone : r1415-1)
|Gene ID :||3005|
|Uniprot ID :||P07305|
|Alternative Name :||H1(0), H1.1, H1.2, H1.3, H1.4, H1.5, H10, H1A, H1F0, H1F1, H1F2, H1F3, H1F4, H1F5, H1FNT, H1FOO, H1FT, H1FV, H1FX, H1t, H1T2, H1X, HANP1, His1, HisC, HIST1, HIST1H1A, HIST1H1B, HIST1H1C, HIST1H1D, HIST1H1E, HIST1H1T, Oocyte-specific histone H1, Testicular H1 histone|
|Amount :||100 µg|
|Isotype :||Mouse IgG2a, kappa|
|Immunogen Information :||Nuclei of human leukemia biopsy cells|
Eukaryotic histones are basic and water-soluble nuclear proteins that form hetero-octameric nucleosome particles by wrapping 146 base pairs of DNA in a left-handed super-helical turn sequentially to form chromosomal fiber. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form the octamer; formed of two H2A-H2B dimers and two H3-H4 dimers, forming two nearly symmetrical halves by tertiary structure. Over 80% of nucleosomes contain the linker Histone H1, derived from an intronless gene that interacts with linker DNA between nucleosomes and mediates compaction into higher order chromatin. Histones are subject to posttranslational modification by enzymes primarily on their N-terminal tails, but also in their globular domains. Such modifications include methylation, citrullination, acetylation, phosphorylation, sumoylation, ubiquitination and ADP-ribosylation.
|Purification :||Purified Ab with BSA and Azide at 200ug/ml|
|Content :||200ug/ml of recombinant MAb purified by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.|
|Storage condition :||Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.|
MW : ~30kDa; Positive Control : HeLa, A-431, LNCap or Jurkat cells. Breast carcinoma.;Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Nucleus, Chromosome|
|Post transnational modification:||ADP-ribosylated on Ser-104 in response to DNA damage.|
|BioGrid:||109260. 47 interactions.|