TLR4/IL-8 Leeporter™ Luciferase Reporter-HeLa Cell Line
|Amount :||Non Profit|
The TLR4/IL-8 Leeporter™ Luciferase Reporter cell line is a stably transfected HeLa cell line which expresses human TLR4, MD-2 and CD14 as well Renilla luciferase reporter gene under the transcriptional control of the IL-8 promoter. IL-8 is one of the major pro-inflammatory cytokines induced by ligand (such as LPS)-mediated Toll-like receptor 4 (TLR4) activation. TLR4 is one of the key innate immune receptors, which is activated by LPS and can lead to sepsis upon dysregulation. The TLR4/IL-8 activation by LPS is shown in Figure 1.
|Content :||Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO.|
|Storage condition :||Immediately upon receipt, store in liquid nitrogen.|
- Monitor the TLR4 signaling pathway activity.
- Screen for activators or inhibitors of the TLR4 signaling pathway.
To passage the cells, detach cells from culture vessel with Trypsin/EDTA, add complete growth medium and transfer to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cells suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
A. Response of TLR4/IL-8 Leeporter™ – HeLa cells to LPS.
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research. The buyer cannot sell, give or otherwise transfer this product to a third party.
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For Research Use Only. Not for use in diagnostic/therapeutics procedures.