Abeomics Logo
  • (0) Items
    • Items | total: $0.00 (0)
    • Your shopping cart is empty!

Abeomics Logo

Antibodies & Engineered Cell Lines™

  • Account
    • Login
    • Address Book
    • My Order
    • My Wish List
    • Open a New Ticket
  • (0) Items
    • Your shopping cart is empty!

  • About us
  • Products
      • Antibodies
        • TLR and Innate Immunity
        • Apoptosis
        • Immunology
        • DNA methylation and Repair
        • Cell Signalling
        • Infectious Diseases
        • NF-kB Pathway
        • Cancer Marker
        • Loading Controls
        • Stem Cells
        • Development and Differentiation
        • Isotype Controls
        • Secondary Antibodies
        • Tag Antibodies
        • Miscellaneous
      • Cell Lines
        • Primary Cells
        • Reporter Cell Lines
        • Stable Cell Lines
      • Ligands and Inhibitors
        • Peptide Inhibitors
        • Proteases inhibitors
        • TLR Ligands
        • Caspase Inhibitors
      • Recombinant Proteins
      • Immune-Check Point
      • Kits and Reagents
        • Luciferase reporter assay kits
        • Inhibitor Screening Kit
        • ELISA Kits
        • Molecular Biology Kits
        • Apoptosis Detection kits
        • Flowcytometry staining kits
        • Cell dissociation solutions
        • Western Blot membranes (Quick blots/ Q Blots)
      • Tissue Microarray
      • recmAb™
        • Recombinant Biosimilar Antibodies
        • Recombinant Rabbit Antibodies
        • Recombinant Mouse Antibodies
  • Pathways
      • All-Pathways

        View all pathways

      • interactive-pathways

        View all interactive pathways

  • Custom Service
    • Drug Discovery Screening Services
    • Stable Cell Line Development
    • Protein Production
    • Custom Antibody Development
  • Quick order
    • + Add More..

  • Support
  • Contact us
  • Distributors
  1. Catalog
  2. /
  3. Antibodies
  4. /
  5. Loading Controls
  6. /
  7. Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Share:

Figure-1: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 0.5 µg/ml on (1) Panc-1, (2) EL-4, (3) Jurkat and (4) HepG2 lysates.

Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Roll over image to zoom in

   
Figure-1: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 0.5 µg/ml on (1) Panc-1, (2) EL-4, (3) Jurkat and (4) HepG2 lysates.
Figure-2 : Immunohistochemical analysis of GAPDH in human Liver tissue using GAPDH antibody (Clone: ABM22C5) at 3 µg/ml.
Figure-3: Immunohistochemical analysis of GAPDH in human Small intestine tissue using GAPDH antibody (Clone: ABM22C5) at 10 µg/ml.
Figure-4: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on (1) CHO-K1 and (2) CHO/PD1 transfected Lysates.
Figure-5: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 4 µg/ml on Raw cell  Lysate.
Figure-6: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Lung, (2) h Testis, (3) h Liver and (4) h Ovary lysates.
Figure-7: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) h Brain, (2) m Brain, (3) h Pancrease, (4) h Kidney and (5) m Skeletal Muscle lysates.
Figure-8: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) PC3, (2) A549, (3)THP1, (4) Ramos, (5) HCT-116, (6) Raji and (7) MOLT-4 lysates.
Figure-9: Western blot analysis of GAPDH. Anti- GAPDH antibody (Clone: ABM22C5) was used at 1 µg/ml on (1) U937, (2) Saos2, (3) A431, (4) K562, (5) U87, (6) MCF-7, (7) Snu1 and (8) Jurkat lysates.

Product code: 10-10011

Clone name : ABM22C5
Clonality : Monoclonal
Application : IHC, WB
Reactivity : Mouse, Human

Shipping Info:

Order now and get it on Tuesday March 02, 2021

Same day delivery FREE on San Diego area orders placed by 1.00 PM

Local delivery areas

Same-day delivery in San Diego available for the following zip codes:

92121, 92122, 92037, 92117, 92109, 92110, 92101, 92010, 92011, 92009, 92008
Download TDS MSDS
Write a review for this product on BioCompare
Get $20 gift card from Amazon
Size
Price

Available Pack Size(s)

  •   25 µg

  •  100 µg

  • $90.00 

  • $270.00 

Add to Wish List

Bulk Order

Shipping Info:

Order now and get it on Tuesday March 02, 2021

Same day delivery FREE on San Diego area orders placed by 1.00 PM

Download TDS MSDS

  • Product Info
  • Antigen Details
  • Description
  • Application Note
  • Citations (18)
  • More
  • Review   (0)
Format : Purified
Amount : 100 µg
Isotype : Mouse IgG1 Kappa
Purification : Protein G Chromatography
Content : 25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : GAPDH
Gene ID : 2597
Uniprot ID : P04406
Alternative Name : GAPDH, GAPD, CDABP0047, OK/SW-cl.12
Immunogen Information : A partial length recombinant GAPDH protein (amino acids 120-320) was used as the immunogen for this antibody.

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) is an enzyme best known for its role in glycolysis. However, extra-glycolytic functions of GAPDH have been described, including regulation of protein expression via RNA binding. GAPDH binds to numerous AREs (adenine-uridine rich elements) from various mRNA 3'-untranslated regions in vitro and in vivo despite its lack of a canonical RNA binding motif. GAPDH specifically catalyzes the simultaneous phosphorylation and oxidation of glyceraldehyde 3-phosphate using NAD+ (Nicotinamide Adenine Dinucleotide) as a cofactor to produce glycerate 1,3-biphosphate and NADH. In addition to its role in energy production, GAPDH has been implicated in many cellular processes including DNA repair tRNA export, membrane fusion and transport, endocytosis and nuclear membrane assembly, and cell death.

Western blot analysis: 0.5-2 µg/ml, Immunohistochemical analysis: 5 µg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

1. Kakade PS, Budnar S, Kalraiya RD, Vaidya MM. Functional Implications of O-GlcNAcylation-dependent Phosphorylation at a Proximal Site on Keratin 18. J Biol Chem. 2016 Jun 3;291(23):12003-13. doi: 10.1074/jbc.M116.728717. Epub 2016 Apr 8. PubMed PMID: 27059955.

2. Rohit Shetty, Anupam Sharma, Natasha Pahuja, Priyanka Chevour, Neeraja Padmajan, Kamesh Dhamodaran, Chaitra Jayadev, Rudy M. M. A. Nuijts, Arkasubhra Ghosh, Jeyabalan Nallathambi. Oxidative stress induces dysregulated autophagy in corneal epithelium of keratoconus patients. PLoS One. 2017; 12(9): e0184628. Published online 2017 Sep 13. doi: 10.1371/journal.pone.0184628 PMCID: PMC5597215

3. Ashraf R, Hamidullah, Hasanain M, Pandey P, Maheshwari M, Singh LR, Siddiqui MQ, Konwar R, Sashidhara KV, Sarkar J. Coumarin-chalcone hybrid instigates DNA damage by minor groove binding and stabilizes p53 through post translational modifications. Sci Rep. 2017 Mar 28;7:45287. doi: 10.1038/srep45287. PubMed PMID: 28349922; PubMed Central PMCID: PMC5368660.

4. Kumar S, Singh U, Goswami C, Singru PS. Transient receptor potential vanilloid 5 (TRPV5), a highly Ca(2+) -selective TRP channel in the rat brain: relevance to neuroendocrine regulation. J Neuroendocrinol. 2017 Apr;29(4). doi: 10.1111/jne.12466. PubMed PMID: 28235149.

5. Sahay P, Rao A, Padhy D, Sarangi S, Das G, Reddy MM, Modak R. Functional Activity of Matrix Metalloproteinases 2 and 9 in Tears of Patients With Glaucoma. Invest Ophthalmol Vis Sci. 2017 May 1;58(6):BIO106-BIO113. doi: 10.1167/iovs.17-21723. PubMed PMID: 28586796.

6. Nayak TK, Mamidi P, Kumar A, Singh LP, Sahoo SS, Chattopadhyay S, Chattopadhyay S. Regulation of Viral Replication, Apoptosis and Pro-Inflammatory Responses by 17-AAG during Chikungunya Virus Infection in Macrophages. Viruses. 2017 Jan 6;9(1). pii: E3. doi: 10.3390/v9010003. PubMed PMID: 28067803; PubMed Central PMCID: PMC5294972.

7. Panigrahi B, Singh RK, Mishra S, Mandal D. Cyclic peptide-based nanostructures as efficient siRNA carriers. Artif Cells Nanomed Biotechnol. 2018 Oct 12:1-11. doi: 10.1080/21691401.2018.1511574. [Epub ahead of print] PubMed PMID: 30311806.

8. Chloroquine Protects Human Corneal Epithelial Cells from Desiccation Stress Induced Inflammation without Altering the Autophagy Flux. S Shivakumar, T Panigrahi, R Shetty, M Subramani, A Ghosh, N Jeyabalan. BioMed Research International, 2018.

9. Pandey P, Singh D, Hasanain M, Ashraf R, Maheshwari M, Choyal K, Singh A, Datta D, Kumar B, Sarkar J. 7-hydroxyfrullanolide, isolated from Sphaeranthus indicus, inhibits colorectal cancer cell growth by p53 dependent and independent mechanism. Carcinogenesis. 2018 Dec 8. doi: 10.1093/carcin/bgy176. [Epub ahead of print] PubMed PMID: 30535334.

10. Panigrahi B, Mishra S, Singh RK, Siddiqui N, Bal R, Mandal D. Peptide generated anisotropic gold nanoparticles as efficient siRNA vectors. Int J Pharm. 2019 May 30;563:198-207. doi: 10.1016/j.ijpharm.2019.04.007. Epub 2019 Apr 3. PubMed PMID: 30953762.

11. Anuja K, Chowdhury AR, Saha A, Roy S, Rath AK, Kar M, Banerjee B. Radiation-induced DNA damage response and resistance in colorectal cancer stem-like cells. Int J Radiat Biol. 2019 Jun;95(6):667-679. doi: 10.1080/09553002.2019.1580401. Epub 2019 Mar 20. PubMed PMID: 30753097.

12. Mishra S, Verma SS, Rai V, Awasthee N, Arya JS, Maiti KK, Gupta SC. Curcuma raktakanda Induces Apoptosis and Suppresses Migration in Cancer Cells: Role of Reactive Oxygen Species. Biomolecules. 2019 Apr 23;9(4). pii: E159. doi: 10.3390/biom9040159. PubMed PMID: 31018580; PubMed Central PMCID: PMC6523773.

13. Nayak TK, Mamidi P, Sahoo SS, Kumar PS, Mahish C, Chatterjee S, Subudhi BB, Chattopadhyay S, Chattopadhyay S. P38 and JNK Mitogen-Activated Protein Kinases Interact With Chikungunya Virus Non-structural Protein-2 and Regulate TNF Induction During Viral Infection in Macrophages. Front Immunol. 2019 Apr 12;10:786. doi: 10.3389/fimmu.2019.00786. eCollection 2019. PubMed PMID: 31031770; PubMed Central PMCID: PMC6473476.

14. Hayat B, Padhy B, Mohanty PP, Alone DP. Altered unfolded protein response and proteasome impairment in pseudoexfoliation pathogenesis. Exp Eye Res. 2019 Apr;181:197-207. doi: 10.1016/j.exer.2019.02.004. Epub 2019 Feb 7. PubMed PMID: 30738879.

15. Chaudhary P, Babu GS, Sobti RC, Gupta SK. HGF regulate HTR-8/SVneo trophoblastic cells migration/invasion under hypoxic conditions through increased HIF-1α expression via MAPK and PI3K pathways. J Cell Commun Signal. 2019 Jan 26. doi: 10.1007/s12079-019-00505-x. [Epub ahead of print] PubMed PMID: 30684191.

16. Singh O, Pradhan DR, Nagalakashmi B, Kumar S, Mitra S, Sagarkar S, Sakharkar AJ, Lechan RM, Singru PS. Thyrotropin-releasing hormone (TRH) in the brain and pituitary of the teleost, Clarias batrachus and its role in regulation of hypophysiotropic dopamine neurons. J Comp Neurol. 2019 Apr 15;527(6):1070-1101. doi: 10.1002/cne.24570. Epub 2018 Dec 28. PubMed PMID: 30370602.

17. Kokate SB, Dixit P, Das L, Rath S, Roy AD, Poirah I, Chakraborty D, Rout N, Singh SP, Bhattacharyya A. Acetylation-mediated Siah2 stabilization enhances PHD3 degradation in Helicobacter pylori-infected gastric epithelial cancer cells. FASEB J. 2018 Oct;32(10):5378-5389. doi: 10.1096/fj.201701344RRR. Epub 2018 Apr 24. PubMed PMID: 29688807.

18. Padhy B, Kapuganti RS, Hayat B, Mohanty PP, Alone DP. De novo variants in an extracellular matrix protein coding gene, fibulin-5 (FBLN5) are associated with pseudoexfoliation. Eur J Hum Genet. 2019 Jul 29. doi: 10.1038/s41431-019-0482-6. [Epub ahead of print] PubMed PMID: 31358954.

Subcellular location: Cytoplasm, Nucleus, Cytoplasm, Membrane, Cytoplasm
Post transnational modification: Oxidative stress can promote the formation of high molecular weight disulfide-linked GAPDH aggregates, through a process called nucleocytoplasmic coagulation. Such aggregates can be observed in vivo in the affected tissues of patients with Alzheimer disease or alcoholic liver cirrhosis, or in cell cultures during necrosis. Oxidation at Met-46 may play a pivotal role in the formation of these insoluble structures. This modification has been detected in vitro following treatment with free radical donor (+/-)-(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide. It has been proposed to destabilize nearby residues, increasing the likelihood of secondary oxidative damages, including oxidation of Tyr-45 and Met-105. This cascade of oxidations may augment GAPDH misfolding, leading to intermolecular disulfide cross-linking and aggregation.
BioGrid: 108868. 233 interactions.
There are currently no product reviews
Write a review on this product!

Customers who purchased this product also purchased

Anti-BRCA1 (Breast Marker) Monoclonal Antibody(Clone: BRCA1/2986)

Anti-BRCA1 (Breast Marker) Mon...

details-Anti-BRCA1 (Breast Marker) Monoclonal Antibody(Clone: BRCA1/2986)
Coronavirus (COVID-19) Spike Receptor Binding Domain (300-600 a.a.)

Coronavirus (COVID-19) Spike R...

details-Coronavirus (COVID-19) Spike Receptor Binding Domain (300-600 a.a.)
Anti-Cyclin B2 Monoclonal Antibody(Clone: X29.2)

Anti-Cyclin B2 Monoclonal Anti...

details-Anti-Cyclin B2 Monoclonal Antibody(Clone: X29.2)
Anti-Calbindin 1 (CALB1) Monoclonal Antibody(Clone: CALB1/3333)

Anti-Calbindin 1 (CALB1) Monoc...

details-Anti-Calbindin 1 (CALB1) Monoclonal Antibody(Clone: CALB1/3333)
Anti-BMI1 (Oncogene and Stem Cell Marker) Monoclonal Antibody(Clone: BMI1/2689)

Anti-BMI1 (Oncogene and Stem C...

details-Anti-BMI1 (Oncogene and Stem Cell Marker) Monoclonal Antibody(Clone: BMI1/2689)
Monoclonal Antibody to Rap1 (Clone: ABM13D9)

Monoclonal Antibody to Rap1 (C...

details-Monoclonal Antibody to Rap1 (Clone: ABM13D9)
Anti-Calnexin (Endoplasmic Reticulum Marker) Monoclonal Antibody(Clone: CANX/1543)

Anti-Calnexin (Endoplasmic Ret...

details-Anti-Calnexin (Endoplasmic Reticulum Marker) Monoclonal Antibody(Clone: CANX/1543)
Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody(Clone: 4C6-H8)

Anti-TNF-alpha (Tumor Necrosis...

details-Anti-TNF-alpha (Tumor Necrosis Factor alpha) Monoclonal Antibody(Clone: 4C6-H8)
SARS-CoV-2 Nucleocapsid Antibody (Clone: 1G5)

SARS-CoV-2 Nucleocapsid Antibo...

details-SARS-CoV-2 Nucleocapsid Antibody (Clone: 1G5)
Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal Antibody(Clone: VWF/1465)

Anti-von Willebrand Factor / F...

details-Anti-von Willebrand Factor / Factor VIII Related-Ag (Endothelial Marker) Monoclonal Antibody(Clone: VWF/1465)
Anti-CD20 / MS4A1 (B-Cell Marker) Polyclonal Antibody

Anti-CD20 / MS4A1 (B-Cell Mark...

details-Anti-CD20 / MS4A1 (B-Cell Marker) Polyclonal Antibody

Most viewed Products

Recombinant Human Thioredoxin-Like 4A

Recombinant Human Thioredoxin-Like ...

details-Recombinant Human Thioredoxin-Like 4A
Monoclonal Antibody to Human IL-1beta (Clone: ABM26G5)

Monoclonal Antibody to Human IL-1be...

details-Monoclonal Antibody to Human IL-1beta (Clone: ABM26G5)
Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)

Monoclonal antibody to Human PD-L1 ...

details-Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)
Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)

Monoclonal Antibody to Caspase-3 (P...

details-Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)
Polyclonal Antibody to Beta actin

Polyclonal Antibody to Beta actin

details-Polyclonal Antibody to Beta actin
Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Monoclonal Antibody to GAPDH (Clone...

details-Monoclonal Antibody to GAPDH (Clone: ABM22C5)
Monoclonal antibody to B7-H4 (Clone: ABM53A6)

Monoclonal antibody to B7-H4 (Clone...

details-Monoclonal antibody to B7-H4 (Clone: ABM53A6)
Polyclonal Antibody to Beta Tubulin

Polyclonal Antibody to Beta Tubulin

details-Polyclonal Antibody to Beta Tubulin
NF-kB Leeporter™ Luciferase Reporter-RAW264.7 Cell Line

NF-kB Leeporter™ Luciferase Repor...

details-NF-kB Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
Peroxidase conjugated Goat anti Mouse IgG (H+L)

Peroxidase conjugated Goat anti Mou...

details-Peroxidase conjugated Goat anti Mouse IgG (H+L)

Related Products

Anti-alpha-tubulin Monoclonal Antibody (Clone:TU-02)

Anti-alpha-tubulin Monoclonal Antibody (Clone:TU-02)

Polyclonal Antibody to Beta Tubulin

Polyclonal Antibody to Beta Tubulin

Polyclonal Antibody to Beta actin

Polyclonal Antibody to Beta actin

New Products

Phospho-p53 (Ser15) (Clone: 1C11) rabbit mAb FITC conjugate

Phospho-p53 (Ser15) (Clone: 1C11) rabbit mAb FITC conjugate

Phospho-Ship2 (Tyr1135) (Clone: 1D2) rabbit mAb

Phospho-Ship2 (Tyr1135) (Clone: 1D2) rabbit mAb

Phospho-Akt1 (Ser473) (Clone: C7) rabbit mAb

Phospho-Akt1 (Ser473) (Clone: C7) rabbit mAb

close

Please Login to write a Review !!


close

Monoclonal Antibody to GAPDH (Clone: ABM22C5)

Product code: 10-10011
*specify in mg or ml

Abeomics Logo

Antibodies & Engineered Cell Lines™

Tel : 858-263-4982

Fax : 858-247-7052

Email : support@abeomics.com

Connect with Us

  • Facebook
  • Twitter
  • Linkedin
  • YouTube

Products

  • Antibodies
  • Cell Lines
  • Ligands and Inhibitors
  • Recombinant Proteins
  • Immune-Check Point
  • Kits and Reagents
  • Tissue Microarray
  • recmAb™

Services

  • Drug Discovery Screening Services
  • Stable Cell Line Development
  • Protein Production
  • Custom Antibody Development

Quick order

+ Add More..

Newsletter

Posters and Flyers

© 2021 Abeomics. All rights reserved.
  • Blog
  • Sitemap
  • Terms
  • Privacy Policy
  • Legal
  • Email unsubscribe

Item added to cart successfully

No cart item available
Continue shopping View Cart