Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)

Fig-1: Western blot analysis of Caspase-3. Anti- Caspase-3 (Clone: ABM1C12) was used at 2 µg/ml on Ramos lysate.

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Fig-1: Western blot analysis of Caspase-3. Anti- Caspase-3 (Clone: ABM1C12) was used at 2 µg/ml on Ramos lysate.

Fig-2 : Immunohistochemical analysis of Caspase-3 in human colon tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.

Fig-3 :Immunohistochemical analysis of Caspase-3 in adenocarcinoma of rectum using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.

Fig-4 :Immunohistochemical analysis of Caspase-3 in human spleen tissue using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.

Fig-5 :Immunohistochemical analysis of Caspase-3 in squamous cell carcinoma of lungs using Caspase-3 antibody (Clone: ABM1C12) at 5 µg/ml.

Fig-6 :Immunohistochemical analysis of Caspase-3 in human Small Intestine tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.

Fig-7 :Immunohistochemical analysis of Caspase-3 in human Tonsil tissue using Caspase-3 antibody (Clone: ABM1C12) at 10 µg/ml.

Fig-8: Intracellular FLOW cytometric analysis of Caspase 3 (Clone : ABM1C12) inon Jurkat cells using 05 µg/10^6 cells of antibody. Goat anti-mouse PE conjugate was used as secondary antibody. Green represents isotope control (ABEOMICS), red represents anti-caspase 3 antibody.

Product code: 10-1003

Clone name :	ABM1C12
Application :	IHC, FACS, WB
Reactivity :	Human

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25 µg
100 µg

$90.00
$260.00

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Order now and get it on Tuesday April 24, 2018

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Download TDS MSDS


Gene :	Casp3
Gene ID :	836
Uniprot ID :	P42574
Alternative Name :	Casp3, Cpp32
Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG1 Kappa
Immunogen Information :	Full length recombinant Caspase-3 protein was used as the immunogen for this antibody.

Caspases are a member of the cysteine-aspartic acid protease family. Caspase-3 (31 kDa) is an executionary caspase which directly cleaves and activates poly(ADP-ribose) polymerase (PARP), sterol regulatory element binding proteins (SREBPs) or it can also interact with other caspases like caspase-6, -7 and -9. Increased levels of caspase-3 are involved in Huntington Disease-associated cell death. Caspase-3 is the principal caspase in mediating the cleavage of amyloid-beta 4A precursor protein (APP), which is related with neuronal death in Alzheimer's disease. Like other caspases, caspase-3 is also synthesized as a zymogen procaspase which is activated by specific proteolytic cleavage. High levels of caspase-3 are observed in lung, spleen, heart, liver and kidney, moderate levels in brain and skeletal muscle, and low in testis.

Purification :	Protein G Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western blot analysis: 2-4 µg/ml, Immunohistochemical analysis: 5-10 µg/ml, FACS analysis: 0.5-1 µg/10^6 cells

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm
Post transnational modification:	S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol.
Tissue Specificity:	Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
BioGrid:	107286. 100 interactions.