Polyclonal Antibody to Beta actin

Fig-1: Western blot analysis of Beta actin. Anti- Beta actin antibody (11-13012) was used at 4 µg/ml on 1) Raw, 2) 3T3, 3) Hela and 4) U87 lysates.

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Fig-1: Western blot analysis of Beta actin. Anti- Beta actin antibody (11-13012) was used at 4 µg/ml on 1) Raw, 2) 3T3, 3) Hela and 4) U87 lysates.

Product code: 11-13012

Application :	WB
Reactivity :	Mouse, Human

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25 µg
100 µg

$80.00
$240.00

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Order now and get it on Tuesday April 24, 2018

Same day delivery FREE on San Diego area orders placed by 1.00 PM

Download TDS MSDS


Gene :	ACTB
Gene ID :	60
Uniprot ID :	P60709
Alternative Name :	Actin, cytoplasmic 1, ACTB
Format :	Purified
Amount :	100 µg
Isotype :	Rabbit IgG
Immunogen Information :	A partial length recombinant Beta actin protein (amino acids 1-200) was used as the immunogen for this antibody.

B-actin (ACTB) has traditionally been regarded as an endogenous housekeeping gene and has been widely used as a reference gene/protein in quantifying expression levels in tumors. It supports fundamental cellular processes in healthy and diseased cells including cell adhesion, migration, cytokinesis and maintenance of cell polarity. However, B-actin is closely associated with a variety of cancers and accumulating evidence indicates that B-actin is de-regulated in liver, melanoma, renal, colorectal, gastric, pancreatic, esophageal, lung, breast, prostate, ovarian cancers, leukemia and lymphoma. This protein is generally found to be up-regulated in the majority of tumor cells and tissues.

Purification :	Protein A Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.

Western blot analysis: 4-6 µg/ml:

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cytoplasm
Post transnational modification:	(Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-50 of one monomer and Glu-270 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding (PubMed:19015515). The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners (PubMed:26228148).
BioGrid:	106575. 305 interactions.