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Fig-1: Western blot analysis of Beta actin. Anti- Beta actin antibody (11-13012) was used at 4 µg/ml on 1) Raw, 2) 3T3, 3) Hela and 4) U87 lysates.
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B-actin (ACTB) has traditionally been regarded as an endogenous housekeeping gene and has been widely used as a reference gene/protein in quantifying expression levels in tumors. It supports fundamental cellular processes in healthy and diseased cells including cell adhesion, migration, cytokinesis and maintenance of cell polarity. However, B-actin is closely associated with a variety of cancers and accumulating evidence indicates that B-actin is de-regulated in liver, melanoma, renal, colorectal, gastric, pancreatic, esophageal, lung, breast, prostate, ovarian cancers, leukemia and lymphoma. This protein is generally found to be up-regulated in the majority of tumor cells and tissues.
Western blot analysis: 4-6 µg/ml:
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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