Anti-5-bromodeoxyuridine (BrdU)-Biotin Conjugated Monoclonal Antibody (Clone:Bu20a)
Figure 1: Flow cytometry intracellular staining pattern of BrdU incorporated K562 cells stained using anti-BrdU (Bu20a) Biotin antibody (concentration in sample 0.5 μg/ml, Streptavidin APC).
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| Amount : | 0.1 mg |
| Isotype : | Mouse IgG1 |
| Purification : | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Content : | 1 mg/ml; Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4 |
| Storage condition : | Store at 2-8°C. Do not freeze. |
| Gene ID : | 35 |
| Alternative Name : | 5-bromodeoxyuridine, Bu20a |
| Immunogen Information : | Bromodeoxyuridine conjugated to BSA |
Bromodeoxyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU.
Specificity: The antibody Bu20a reacts specifically with BrdU incorporated into DNA during S-phase of a cell cycle. It is useful for detecting proliferating cells by flow cytometry or immunohistochemistry staining. It cross-reacts with EdU.
Flow cytometry: Recommended dilution: 1-5 μg/ml.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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