Anti-alpha-Tubulin Monoclonal Antibody (Clone: YOL1/34)

Figure 1: Western blotting analysis of human alpha-tubulin using rat monoclonal antibody YOL1/34 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rat anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated anti-rat secondary antibody. A specific band was detected for alpha-tubulin at approximately 54 kDa.

Anti-alpha-Tubulin Monoclonal Antibody (Clone: YOL1/34)

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Figure 1: Western blotting analysis of human alpha-tubulin using rat monoclonal antibody YOL1/34 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of rat anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated anti-rat secondary antibody. A specific band was detected for alpha-tubulin at approximately 54 kDa.

Figure 2: Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).

Figure 3: Western blotting analysis of human alpha-tubulin using rat monoclonal antibody YOL1/34 on lysates of Caenorhabditis elegans, Arabidopsis thaliana, Trypanosoma brucei, Drosophila melanogaster, human HeLa cell line, and porcine brain, all under reducing conditions. Each lane contains 20 μg of total protein. Nitrocellulose membrane was probed with 2 µg/ml of rat anti-alpha-tubulin monoclonal antibody followed by IRDye800-conjugated anti-rat secondary antibody.

Figure 4: Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-alpha-tubulin (YOL1/34) purified antibody (concentration in sample 8 μg/ml, GAM APC).

Figure 5: Separation of human monocytes stained using anti-alpha-tubulin (YOL1/34) purified antibody (concentration in sample 8 μg/ml, GAM APC, red-filled) from monocytes stained using mouse IgG1 isotype control (MOPC-21) purified antibody (concentration in sample 8 μg/ml, same as alpha-tubulin purified, GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Product code: 30-2930

Clone name :	YOL1/34
Clonality :	Monoclonal
Application :	ICC, ELISA, ICC/IF, IHC, FACS, WB
Conjugate :	Unconjugated

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For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Format :	Purified
Amount :	0.1 mg
Isotype :	Rat IgG2a
Purification :	Purified by protein-G affinity chromatography.
Content :	1mg/ml, Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage condition :	Store at 2-8°C. Do not freeze.


Gene ID :	7277
Uniprot ID :	Q71U36
Alternative Name :	TUBA
Immunogen Information :	Yeast tubulin

The rat monoclonal antibody YOL1/34 recognizes an epitope of alpha-tubulin localized between amino acids 414-422. It has higher affinity for fixed microtubules than for native ones.

Immunocytochemistry: Recommended dilution 4-8 μg/ml.<br>Western blotting: Recommended dilution 1-2 μg/ml; reducing conditions.<br>Flow cytometry: Recommended dilution: 8-12 μg/ml; intracellular staining.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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