Anti-CD22 Monoclonal Antibody (Clone:MEM-01)
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Format : | Purified |
Amount : | 0.1 mg |
Isotype : | Mouse IgG1 |
Purification : | Purified by protein-A affinity chromatography |
Storage condition : | Store at 2-8°C. Do not freeze. |
Flow Cytometry Recommended dilution: 2-4 µg/ml
Immunoprecipitation Western Blotting Recommended dilution: 2-4 µg/ml, 120 min on vertical incubator
Positive control: RAJI human lymphoma cell line
Sample preparation: Resuspend approx. 50 mil. cells in 1 ml cold Lysis buffer (1% laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Protease inhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysate with non-reducing SDS-PAGE sample buffer.
The antibody MEM-01 stains only the zone corresponding to 140 kDa, but not the weaker 130 kDa zone (stained by several CD22 antibodies recognizing both isoforms).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
Subcellular location: | Cell membrane |
Post transnational modification: | Phosphorylated on tyrosine residues by LYN. |
Tissue Specificity: | B-lymphocytes. |
BioGrid: | 107371. 15 interactions. |
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