Fig. 1: FCM staining of human PBMCs using CD5 Monoclonal Antibody (CRIS-1).
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Product code: 36-3492
| Clone name : | CRIS-1 |
| Application : | FACS, IF, IP |
| Reactivity : | Human, Rhesus Monkey |
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Recognizes a 67kDa transmembrane protein, which is identified as CD5 (HLDA I; WS Code T 29HLDA III; WS Code T 530). The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. Anti-CD5 is a pan T-cell marker that also reacts with a range of neoplastic B-cells, e.g. chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL), mantle cell lymphoma, and a subset (~10%) of diffuse large B-cell lymphoma. CD5 aberrant expression is useful in making a diagnosis of mature T-cell neoplasms.
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1µg/ml); Functional Studies (Order Ab without BSA & Azide); Immunoprecipitation (0.5-1µg/500µg protein lysate); Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
