Anti-CD54 / ICAM-1 Monoclonal Antibody (Clone:1H4) PE-Cy™7 conjugated

Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD54 (1H4) PE-Cy™7 antibody (concentration in sample 0.33 μg/ml).

Anti-CD54 / ICAM-1 Monoclonal Antibody (Clone:1H4) PE-Cy™7 conjugated

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Figure 1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD54 (1H4) PE-Cy™7 antibody (concentration in sample 0.33 μg/ml).

Figure 2: Separation of human monocytes (red-filled) from CD54 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD54 (1H4) PE-Cy™7 antibody (concentration in sample 0.33 μg/ml).

Product code: 30-1018PE-Cy™7

Clone name :	1H4
Clonality :	Monoclonal
Application :	FACS
Reactivity :	Human
Conjugate :	PE/CY7

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Shipping Info:

For estimated delivery dates, please contact us at [email protected]

Download TDS MSDS

Amount :	100 tests
Isotype :	Mouse IgG2b
Purification :	Purified antibody is conjugated with activated tandem dye of R-phycoerythrin-cyanine 7 (PE-Cy™7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Content :	Formulation: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage condition :	Store at 2-8°C. Do not freeze.


Gene :	ICAM1
Gene ID :	3383
Uniprot ID :	P05362
Alternative Name :	ICAM1,ICAM-1, BB2, P3.58
Immunogen Information :	Raji cells and spleen cells fused with NS1 cells

Specificity: The antibody 1H4 recognizes an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein (receptor for rhinovirus) expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.

Description: CD54 (ICAM-1) is a 90 kD member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1). The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.

Flow Cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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