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  7. Anti-Human CD273 APC (Clone : 24F.10C12)

Anti-Human CD273 APC (Clone : 24F.10C12)

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Figure 1 : Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) PE-Cy™7 antibody (4 µl reagent per milion cells in 100 µl of cell suspension).

Anti-Human CD273 APC (Clone : 24F.10C12)
Anti-Human CD273 APC (Clone : 24F.10C12)

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Figure 1 : Flow cytometry surface staining pattern of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD206 (15-2) PE-Cy™7 antibody (4 µl reagent per milion cells in 100 µl of cell suspension).
Figure 2 : Separation of human dendritic cells differentiated upon monocyte stimulation (GM-CSF + IL-4) (red-filled) from non-stimulated lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human stimulated (GM-CSF + IL-4) peripheral blood mononuclear cells stained using anti-human CD273 (24F.10C12) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Product code: 30-2602

Clone name : 24F.10C12
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : APC

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100 tests
$443.00 

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  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Amount : 100 tests
Isotype : Mouse IgG2a kappa
Purification : The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Gene : PDCD1LG2
Gene ID : 80380
Alternative Name : PDCD1LG2, B7DC, Btdc, PDL2, PDCD1L2, bA574F11.2, Butyrophilin, B7-DC,programmed cell death 1 ligand 2
Immunogen Information : human CD273
CD273 / PD-L2 (programmed death ligand-1), also known as B7-DC, is a member of the B7 family of regulatory proteins. It costimulates the proliferation of T cells, and mediates IFN gamma production. Ligation of CD273 on dendritic cells enhances dendritic cell activation and T cell responses. When interacting with CD279, it can act as a coinhibitor of the T cell function. CD273 expression is a useful marker to distinguish primary mediastinal B cell lymphoma from other diffuse large B cell lymphomas.
Specificity : The mouse monoclonal antibody 24F.10C12 recognizes an extracellular epitope of CD273 / PD-L2 (also known as B7-DC), a 25 kDa type I transmembrane protein expressed by dendritic cells, activated monocytes and T cells, heart, first trimester placenta, lung and liver, as well as in Hodgkin´s lymphoma cells and primary mediastinal B cell lymphoma (PMBL).

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Human CD273 APC (Clone : 24F.10C12)

Product code: 30-2602
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