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  7. Anti-Human CD45 APC (Clone : 2D1)

Anti-Human CD45 APC (Clone : 2D1)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Anti-Human CD45 APC (Clone : 2D1)
Anti-Human CD45 APC (Clone : 2D1)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD45 (2D1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).
Figure 2 : Separation of human CD45 positive lymphocytes (red-filled) from human CD45 negative blood debris (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD45 (2D1) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Product code: 30-2563

Clone name : 2D1
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : APC

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100 tests
$443.00 

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  • Product Info
  • Antigen Details
  • Description
  • Application Note
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Amount : 100 tests
Isotype : Mouse IgG1 kappa
Purification : The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Gene : PTPRC
Gene ID : 5788
Alternative Name : LCA, T200, LY5, B220, GP180, TPC,protein tyrosine phosphatase receptor type C
Immunogen Information : Human peripheral blood mononuclear cells
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Specificity : The mouse monoclonal antibody 2D1 reacts with an extracellular epitope of all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Human CD45 APC (Clone : 2D1)

Product code: 30-2563
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