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  7. Anti-Human CD8 APC (Clone : LT8)

Anti-Human CD8 APC (Clone : LT8)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD8 (LT8) APC antibody (4 µl reagent / 100 µl of peripheral whole blood)

Anti-Human CD8 APC (Clone : LT8)
Anti-Human CD8 APC (Clone : LT8)

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Figure 1 : Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD8 (LT8) APC antibody (4 µl reagent / 100 µl of peripheral whole blood)
Figure 2 : Separation of human CD8 positive lymphocytes (red-filled) from CD8 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD8 (LT8) APC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Product code: 30-2546

Clone name : LT8
Clonality : Monoclonal
Application : FACS
Reactivity : Human
Conjugate : APC

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100 tests
$443.00 

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  • Antigen Details
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Amount : 100 tests
Isotype : Mouse IgG1
Purification : The purified antibody is conjugated with allophycocyanin (APC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Content : Formulation : Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage condition : Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light.
Gene : CD8A
Gene ID : 925
Alternative Name : p32, LEU2
Immunogen Information : human blood lymphocytes
The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Specificity : The mouse monoclonal antibody LT8 recognizes an extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. This antibody blocks Leu2 binding.

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 µl reagent / 100 µl of whole blood or 106 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Human CD8 APC (Clone : LT8)

Product code: 30-2546
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