Anti-MSLN antibody(DM72), Rabbit mAb

Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human MSLN protein, mFc-His tagged protein can bind Rabbit anti-MSLN monoclonal antibody (clone: DM72) in a linear range of 1-100 ng/ml.

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Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human MSLN protein, mFc-His tagged protein can bind Rabbit anti-MSLN monoclonal antibody (clone: DM72) in a linear range of 1-100 ng/ml.

Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human mesothelin (B) were surface stained with Rabbit anti-MSLN monoclonal antibody 1µg/ml (clone: DM72) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Figure 3. FACS data of serially titrated Rabbit anti-MSLN monoclonal antibody (clone: DM72) on Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Figure 4. Affinity ranking of different Rabbit anti-MSLN mAb clones by titration of different concentration onto Hela cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Product code: 12-9059

Clone name :	DM72
Clonality :	Monoclonal
Application :	ELISA, FACS
Reactivity :	Human

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For estimated delivery dates, please contact us at support@abeomics.com

Download TDS MSDS

Amount :	100 µg
Isotype :	Rabbit IgG
Purification :	Purified from cell culture supernatant by affinity chromatography
Content :	Preservative: 0.1% Procline 300 Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Storage condition :	Store at -20°C for 12 months (Avoid repeated freezing and thawing)


Alternative Name :	MSLN, Mesothelin, MPF
Immunogen Information :	Recombinant human mesothelin (Glu296-Gly580) produced by using human HEK293 cells

This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed.

Recommended Dilutions ELISA 1/5000-10000;FACS 1/100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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