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  7. Anti-Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody(Clone: V9)

Anti-Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody(Clone: V9)

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Fig. 1: Formalin-fixed, paraffin-embedded human Uterine Carcinoma stained with Vimentin Mouse Monoclonal Antibody (V9).

Anti-Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody(Clone: V9)
Anti-Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody(Clone: V9)
Anti-Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody(Clone: V9)

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Fig. 1: Formalin-fixed, paraffin-embedded human Uterine Carcinoma stained with Vimentin Mouse Monoclonal Antibody (V9).
Fig. 2: SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (V9). Confirmation of Integrity and Purity of Antibody.
Fig. 3: Western Blot Analysis of U-87 cell lysate using Vimentin Mouse Monoclonal Antibody (V9).

Product code: 36-3364

Clone name : V9
Clonality : Monoclonal
Application : WB, IHC
Reactivity : Human

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  •  100 µg

  • $199.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG1, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : VIM
Gene ID : 7431
Uniprot ID : P08670
Alternative Name : VIM
Immunogen Information : Porcine Lens
This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Western Blot (1-2µg/ml);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-Vimentin (Mesenchymal Cell Marker) Monoclonal Antibody(Clone: V9)

Product code: 36-3364
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