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  7. Anti-ZAP-70 Polyclonal Antibody

Anti-ZAP-70 Polyclonal Antibody

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Figure-1: Western blotting analysis of human ZAP70 using rabbit polyclonal antibody PAb (430) on lysates of MOLT-4 cell line and HEK293T/17 cell line (ZAP70 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of rabbit anti-ZAP70 polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for ZAP70 protein at approximately 70 kDa.

Anti-ZAP-70 Polyclonal Antibody

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Product code: 30-1295

Clonality : Polyclonal
Application : WB
Reactivity : Human

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0.1 mg
$383.00 

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Format : Purified
Amount : 0.1 mg
Isotype : Rabbit IgG
Purification : Purified from rabbit serum by affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : ZAP70
Gene ID : 7535
Uniprot ID : P43403
Alternative Name : ZAP70, SRK
Immunogen Information : Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag

The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.

Western blotting: Recommended dilution: 1-2 μg/ml; positive control: MOLT-4 cells, reducing conditions.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cytoplasm, Cell membrane
Post transnational modification: Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
Tissue Specificity: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
BioGrid: 113367. 52 interactions.
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Anti-ZAP-70 Polyclonal Antibody

Product code: 30-1295
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