Abeomics Logo
  • (0) Items
    • Items | total: $0.00 (0)
    • Your shopping cart is empty!

Abeomics Logo

Antibodies & Engineered Cell Lines™

  • Account
    • Login
    • Address Book
    • My Order
    • My Wish List
    • Open a New Ticket
  • (0) Items
    • Your shopping cart is empty!

  • About us
  • Products
      • Antibodies
        • TLR and Innate Immunity
        • Apoptosis
        • Immunology
        • DNA methylation and Repair
        • Cell Signalling
        • Infectious Diseases
        • NF-kB Pathway
        • Cancer Marker
        • Loading Controls
        • Stem Cells
        • Development and Differentiation
        • Isotype Controls
        • Secondary Antibodies
        • Tag Antibodies
        • Miscellaneous
      • Cell Lines
        • Reporter Cell Lines
        • Stable Cell Lines
      • Ligands and Inhibitors
        • Peptide Inhibitors
        • Proteases inhibitors
        • TLR Ligands
        • Caspase Inhibitors
      • Recombinant Proteins
        • Immune-Check Point
        • Kits and Reagents
          • Luciferase reporter assay kits
          • Inhibitor Screening Kit
          • ELISA Kits
          • Molecular Biology Kits
          • Apoptosis Detection kits
          • Flowcytometry staining kits
          • Cell dissociation solutions
          • Western Blot membranes (Quick blots/ Q Blots)
        • Tissue Microarray
        • recmAb™
          • Recombinant Biosimilar Antibodies
          • Recombinant Rabbit Antibodies
          • Recombinant Mouse Antibodies
    • Pathways
        • All-Pathways

          View all pathways

        • interactive-pathways

          View all interactive pathways

    • Custom Service
      • Drug Discovery Screening Services
      • Stable Cell Line Development
      • Protein Production
      • Custom Antibody Development
    • Quick order
      • + Add More..

    • Support
    • Contact us
    • Distributors
    1. Catalog
    2. /
    3. Antibodies
    4. /
    5. Cell Signalling
    6. /
    7. Anti-ZAP-70 Polyclonal Antibody

    Anti-ZAP-70 Polyclonal Antibody

    Share:

    Figure-1: Western blotting analysis of human ZAP70 using rabbit polyclonal antibody PAb (430) on lysates of MOLT-4 cell line and HEK293T/17 cell line (ZAP70 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of rabbit anti-ZAP70 polyclonal antibody followed by IRDye800-conjugated anti-rabbit secondary antibody. A specific band was detected for ZAP70 protein at approximately 70 kDa.

    Anti-ZAP-70 Polyclonal Antibody

    Roll over image to zoom in

       

    Product code: 30-1295

    Clonality : Polyclonal
    Application : WB
    Reactivity : Human

    Shipping Info:

    For estimated delivery dates, please contact us at support@abeomics.com

    Download TDS MSDS
    Write a review for this product on BioCompare
    Get $20 gift card from Amazon
    Size
    Price
    0.1 mg
    $383.00  $306.40 

    Add to Wish List

    Bulk Order

    Shipping Info:

    For estimated delivery dates, please contact us at support@abeomics.com

    Download TDS MSDS

    • Product Info
    • Antigen Details
    • Description
    • Application Note
    • More
    • Review   (0)
    Format : Purified
    Amount : 0.1 mg
    Isotype : Rabbit IgG
    Purification : Purified from rabbit serum by affinity chromatography
    Storage condition : Store at 2-8°C. Do not freeze.
    Gene : ZAP70
    Gene ID : 7535
    Uniprot ID : P43403
    Alternative Name : ZAP70, SRK
    Immunogen Information : Bacterially expressed fusion protein representing C-terminal part (160 amino acids) of human ZAP-70 with histidine tag

    The ZAP-70 (zeta-associated protein of 70 kDa) tyrosine kinase was identified as a tyrosine phosphoprotein that associates with TCR zeta subunit and undergoes tyrosine phosphorylation following TCR stimulation. ZAP-70 is a Syk family tyrosine kinase primarily expressed in T and NK cells that plays an essential role in signaling through the TCR. TCR-mediated activation of T cells is crucial to the immune response. In humans, ZAP-70 gene mutations resulting in lower ZAP-70 protein expression levels or expression of catalytically inactive ZAP-70 proteins, have been identified. ZAP-70 deficiency results in the absence of mature CD8+ T cells and the prevention of TCR-mediated activation of CD4+ T cells, and it can lead to severe combined immunodeficiency. ZAP-70 contains two N-terminal SH2 domains (Src homology domain 2) and a C-terminal kinase domain. During T cell activation, the binding of ZAP-70 SH2 domains to the phosphorylated zeta subunit on the activated TCR complex causes a colocalization with the Lck tyrosine kinase that phosphorylates ZAP-70 on Tyr493 in the activation loop. ZAP-70 autophosphorylates multiple tyrosines in the region between the SH2 domains and the kinase domain, including the binding sites for additional SH2-containing signaling proteins such as SLP76, LAT, Lck, PLCgamma1, Vav, Shc, Ras-GAP, and Abl. ZAP-70-mediated activation of these downstream effectors leads to the release of intracellular calcium stores, and the transcription of interleukin-2 and other genes important for an immune response.

    Western blotting: Recommended dilution: 1-2 μg/ml; positive control: MOLT-4 cells, reducing conditions.

    For Research Use Only. Not for use in diagnostic/therapeutics procedures.

    Subcellular location: Cytoplasm, Cell membrane
    Post transnational modification: Ubiquitinated in response to T cell activation. Deubiquitinated by OTUD7B.
    Tissue Specificity: Expressed in T- and natural killer cells. Also present in early thymocytes and pro/pre B-cells.
    BioGrid: 113367. 52 interactions.
    There are currently no product reviews
    Write a review on this product!

    Customers who purchased this product also purchased

    Recombinant Human Secretoglobin Family 3A Member 2

    Recombinant Human Secretoglobi...

    details-Recombinant Human Secretoglobin Family 3A Member 2
    Monoclonal Antibody to mouse M-CSF

    Monoclonal Antibody to mouse M...

    details-Monoclonal Antibody to mouse M-CSF
    Alkaline phosphatase conjugated Goat anti Rabbit IgG (H+L)

    Alkaline phosphatase conjugate...

    details-Alkaline phosphatase conjugated Goat anti Rabbit IgG (H+L)
    Anti-HSP90 alpha/beta Monoclonal Antibody (Clone : Hyb-K41220A) APC(Discontinued)

    Anti-HSP90 alpha/beta Monoclon...

    details-Anti-HSP90 alpha/beta Monoclonal Antibody (Clone : Hyb-K41220A) APC(Discontinued)
    Biotin Conjugated, Anti-alpha-tubulin Monoclonal Antibody (Clone:TU-01)

    Biotin Conjugated, Anti-alpha-...

    details-Biotin Conjugated, Anti-alpha-tubulin Monoclonal Antibody (Clone:TU-01)
    Anti-IDH1 R132H Monoclonal Antibody (Clone:IHC132)

    Anti-IDH1 R132H Monoclonal Ant...

    details-Anti-IDH1 R132H Monoclonal Antibody (Clone:IHC132)
    Anti-CD95 / Fas Monoclonal Antibody (Clone:UT-1)-Azide free(Discontinued)

    Anti-CD95 / Fas Monoclonal Ant...

    details-Anti-CD95 / Fas Monoclonal Antibody (Clone:UT-1)-Azide free(Discontinued)
    Anti-Human CD34 PE-DyLight® 594 (Clone : 4H11[APG])

    Anti-Human CD34 PE-DyLight® 5...

    details-Anti-Human CD34 PE-DyLight® 594 (Clone : 4H11[APG])
    Anti-Thyrotropin (hTSH) Monoclonal Antibody (Clone:TSH-116)

    Anti-Thyrotropin (hTSH) Monocl...

    details-Anti-Thyrotropin (hTSH) Monoclonal Antibody (Clone:TSH-116)
    Anti-HSP60 Monoclonal Antibody (Clone : LK1) ATTO 488

    Anti-HSP60 Monoclonal Antibody...

    details-Anti-HSP60 Monoclonal Antibody (Clone : LK1) ATTO 488
    Anti-Human HER-2 (Trastuzumab) – Fc Muted™ Biotin

    Anti-Human HER-2 (Trastuzumab)...

    details-Anti-Human HER-2 (Trastuzumab) – Fc Muted™ Biotin
    Rat IgG2b Isotype Control Antibody (Clone : S193)

    Rat IgG2b Isotype Control Anti...

    details-Rat IgG2b Isotype Control Antibody (Clone : S193)
    Monoclonal Antibody to CD11c (Clone: ABM4C58)

    Monoclonal Antibody to CD11c (...

    details-Monoclonal Antibody to CD11c (Clone: ABM4C58)
    Anti-Myogenin Monoclonal Antibody (Clone:IHC631)

    Anti-Myogenin Monoclonal Antib...

    details-Anti-Myogenin Monoclonal Antibody (Clone:IHC631)

    Most viewed Products

    Recombinant Human Thioredoxin-Like 4A

    Recombinant Human Thioredoxin-Like ...

    details-Recombinant Human Thioredoxin-Like 4A
    Monoclonal Antibody to Human IL-1beta (Clone: ABM26G5)

    Monoclonal Antibody to Human IL-1be...

    details-Monoclonal Antibody to Human IL-1beta (Clone: ABM26G5)
    NF-kB Leeporter™ Luciferase Reporter-RAW264.7 Cell Line

    NF-kB Leeporter™ Luciferase Repor...

    details-NF-kB Leeporter™ Luciferase Reporter-RAW264.7 Cell Line
    Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)

    Monoclonal antibody to Human PD-L1 ...

    details-Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)
    Polyclonal Antibody to Beta actin

    Polyclonal Antibody to Beta actin

    details-Polyclonal Antibody to Beta actin
    Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)

    Monoclonal Antibody to Caspase-3 (P...

    details-Monoclonal Antibody to Caspase-3 (Pro and Active) (Clone: ABM1C12)
    Monoclonal Antibody to GAPDH (Clone: ABM22C5)

    Monoclonal Antibody to GAPDH (Clone...

    details-Monoclonal Antibody to GAPDH (Clone: ABM22C5)
    Monoclonal antibody to B7-H4 (Clone: ABM53A6)

    Monoclonal antibody to B7-H4 (Clone...

    details-Monoclonal antibody to B7-H4 (Clone: ABM53A6)
    Polyclonal Antibody to Beta Tubulin

    Polyclonal Antibody to Beta Tubulin

    details-Polyclonal Antibody to Beta Tubulin
    Peroxidase conjugated Goat anti Mouse IgG (H+L)

    Peroxidase conjugated Goat anti Mou...

    details-Peroxidase conjugated Goat anti Mouse IgG (H+L)

    Related Products

    Phospho-SEK1/MKK4 (Ser257) (Clone: C5) rabbit mAb

    Phospho-SEK1/MKK4 (Ser257) (Clone: C5) rabbit mAb

    Anti-HSP47 Monoclonal Antibody (Clone : 1C4-1A6) - HRP

    Anti-HSP47 Monoclonal Antibody (Clone : 1C4-1A6) - HRP

    Phospho-MET(Tyr1234/1235) (Clone: 6F11) rabbit mAb APC conjugate

    Phospho-MET(Tyr1234/1235) (Clone: 6F11) rabbit mAb APC conjugate

    close

    Please Login to write a Review !!


    close

    Anti-ZAP-70 Polyclonal Antibody

    Product code: 30-1295
    *specify in mg or ml

    Abeomics Logo

    Antibodies & Engineered Cell Lines™

    Tel : 858-263-4982

    Fax : 858-247-7052

    Email : support@abeomics.com

    Connect with Us

    • Facebook
    • Twitter
    • Linkedin
    • YouTube

    Products

    • Antibodies
    • Cell Lines
    • Ligands and Inhibitors
    • Recombinant Proteins
    • Immune-Check Point
    • Kits and Reagents
    • Tissue Microarray
    • recmAb™

    Services

    • Drug Discovery Screening Services
    • Stable Cell Line Development
    • Protein Production
    • Custom Antibody Development

    Quick order

    + Add More..

    Newsletter

    Posters and Flyers

    © 2022 Abeomics. All rights reserved.
    • Blog
    • Sitemap
    • Terms
    • Privacy Policy
    • Legal
    • Email unsubscribe

    Item added to cart successfully

    No cart item available
    Continue shopping View Cart