DKK3 Human, Sf9
|Alternative Name :||Dickkopf 3 homolog (Xenopus laevis), dickkopf-related protein 3, regulated in glioma, RIG, RIG-like 7-1, RIG-like 5-6, Dkk-3, REIC.|
|Amount :||5 µg|
Sterile Filtered colorless solution.
Dickkopf-related protein 3 (DKK3) belongs to the DKK protein family including Dkk-1, 2, 3 and -4. DKK3 is a 350 amino acid secreted glycoprotein which is comprised of an N-terminal signal peptide and 2 conserved cysteine-rich domains that are separated by a 12 amino acid linker region. DKK3 is involved in embryonic development through its inhibition of the WNT signaling pathway. DKK3 gene expression is decreased in a variety of cancer cell lines and it may act as a tumor suppressor gene.
DKK3 Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 338 amino acids (22-350a.a.) and having a molecular mass of 37.3kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa).DKK3 is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
|Purification :||Greater than 90% as determined by SDS-PAGE.|
|Content :||DKK3 protein solution (0.5mg/ml) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.|
|Storage condition :||Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.|
|Amino acid sequence :||ADPAPAPTAT SAPVKPGPAL SYPQEEATLN EMFREVEELM EDTQHKLRSA VEEMEAEEAA AKASSEVNLA NLPPSYHNET NTDTKVGNNT IHVHREIHKI TNNQTGQMVF SETVITSVGD EEGRRSHECI IDEDCGPSMY CQFASFQYTC QPCRGQRMLC TRDSECCGDQ LCVWGHCTKM ATRGSNGTIC DNQRDCQPGL CCAFQRGLLF PVCTPLPVEG ELCHDPASRL LDLITWELEP DGALDRCPCA SGLLCQPHSH SLVYVCKPTF VGSRDQDGEI LLPREVPDEY EVGSFMEEVR QELEDLERSL TEEMALREPA AAAAALLGGE EIHHHHHH.|