G alpha 15 Stable Cell Line-GPR68-CHO-K1-Human
Figure 1: H+-induced concentration-dependent stimulation of intracellular calcium mobilization in CHO-K1/GPR68/G alpha 15. The cells were loaded with Calcium-4 prior to stimulation with a GPR68 receptor agonist, H+. The intracellular calcium change was measured by FLIPR. The relative fluorescent units (RFU) were plotted against the log of the cumulative doses of H+ (Mean ± SD, n = 2). The EC50 of H+ on GPR68 co-expressing with G alpha 15 in CHO-K1 cells was pH 6.29 (n=1 test) and pH6.66 (n=2 test).
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|Alternative Name :||GPR68; MGC111379; OGR1|
|Amount :||2 vials|
|Content :||Each Vial contains cells in 45% culture medium, 45% FBS, 10% DMSO|
|Storage condition :||Immediately upon receipt, store in liquid nitrogen.|
Application: Screen for antibodies through Flow Cytometry, Immunocytochemistry or Western blotting. Culture Medium: Ham's F12, 10% FBS, 100 µg/ml Hygromycin B, 400 µg/ml G418
For Research Use Only. Not for use in diagnostic/therapeutics procedures.