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  5. GM2A Recombinant Protein

GM2A Recombinant Protein

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GM2A Recombinant Protein

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Product code: 32-3894

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10 µg
$363.00 

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Amount : 10 µg
Purification : Greater than 95.0% as determined by SDS-PAGE.
Content : GM2A filtered (0.4µm) and lyophilized from 0.5mg/ml in 0.05M Tris buffer and 0.1M NaCl, pH-8.0.
Storage condition : Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.
AA sequence : MKHHHHHHAS FSWDNCDEGK DPAVIRSLTL EPDPIIVPGN VTLSVMGSTS VPLSSPLKVD LVLEKEVAGL WIKIPCTDYI GSCTFEHFCD VLDMLIPTGE PCPEPLRTYG LPCHCPFKEG TYSLPKSEFV VPDLELPSWL TTGNYRIESV LSSSGKRLGC IKIAASLKGI.
Alternative Name : Ganglioside GM2 activator, Cerebroside sulfate activator protein, GM2-AP, Sphingolipid activator protein 3, SAP-3, GM2A.

Source : Escherichia Coli. GM2A Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (a.a 33-193) containing 170 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 18.7kDa (calculated). Ganglioside GM2 activator (GM2A) is a small glycolipid transport protein which functions as a substrate specific co-factor for the lysosomal enzyme beta-hexosaminidase A. GM2A participates in lipid metabolism. GM2A protein is localized in lysosomes. The GM2A protein forms a large binding pocket for a number of single chain phospholipids and fatty acids. GM2A demonstrates calcium-independent phospholipase activity. GM2A stimulates ganglioside GM2 degradation by presenting GM2 to beta-hexosaminidase A. The Beta-hexosaminidase A, along with GM2A, catalyzes the degradation of the ganglioside GM2, and other molecules containing terminal N-acetyl hexosamines. GM2A gene mutations result in GM2-gangliosidosis type AB or the AB variant of Tay-Sachs disease.

It is recommended to add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. GM2A is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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GM2A Recombinant Protein

Product code: 32-3894
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