mIL 21 Recombinant Protein
|Amount :||10 µg|
|Purification :||Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.|
|Content :||Mouse IL-21 was lyophilized from 20mM NaHCO3, pH 8.5.|
|Storage condition :||Lyophilized Mouse IL-21 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Mouse IL21 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.|
|AA sequence :||The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Lys-Ser-Ser.|
|Alternative Name :||Interleukin-21, IL-21, Il21.|
Source : Escherichia Coli. Interleukin-21 Mouse Recombinant produced in E.Coli is a single, non-glycosilated polypeptide chain containing 130 amino acids and having a total molecular mass of 15kDa. The Murine IL-21 is purified by proprietary chromatographic techniques. IL-21 is produced by CD4+ T cells in response to antigenic stimulation. Its action enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated B-cells into plasma cells and memory B-cells, stimulation (in conjuction) with IL-4 of IgG production, and induction of apoptotic effects in naive B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (C) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
It is recommended to reconstitute the lyophilized Mouse IL21 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions. The ED50=10 -50 ng/ml, determined by the cell proliferation assay of modified Ba/F3 cells.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.