Anti-beta-Actin Monoclonal Antibody (Clone: AC-15)
Figure 1. Western blot analysis of beta-Actin using anti-beta-Actin antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50µg of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: monkey kidney tissue lysates, Lane 4: monkey liver tissue lysates, Lane 5: rat brain tissue lysates, Lane 6: rat PC-12 whole cell lysates, Lane 7: mouse brain tissue lysates, Lane 8: mouse ANA-1 whole cell lysates, Lane 9: rabbit intestine tissue lysates, Lane 10: rabbit intestine tissue lysates, Lane 11: pig intestine tissue lysates.
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| Amount : | 100 μg/vial |
| Isotype : | Mouse IgG1 |
| Purification : | Ascites |
| Content : | Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative. Reconstitute : Add 1ml of PBS buffer will yield a concentration of 100ug/ml. |
| Storage condition : | At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time. Avoid repeated freezing and thawing. |
| Gene : | Actb |
| Gene ID : | 81822 |
| Uniprot ID : | P60711 |
| Alternative Name : | Actin, cytoplasmic 1; Beta-actin; Actin, cytoplasmic 1, N-terminally processed; Actb |
| Immunogen Information : | slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH. |
The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al.(1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.
Western blot : 0.25-0.5μg/mlμg/ml; Immunohistochemistry(Paraffin-embedded Section) : 0.5-1μg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm |
| Post transnational modification: | Monomethylation at Lys-84 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration (By similarity). |
| BioGrid: | 249680. 12 interactions. |
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