Monoclonal Antibody to CD31 (ABM3E63) FITC Conjugated

Fig.1: FLOW Cytometry analysis of CD31FITC in human PBMC using 0.2µg antibody per 10^6 cells. Green represents isotype control. Red represents FITC conjugated anti-CD31 antibody (Clone: ABM3E63).

Monoclonal Antibody to CD31 (ABM3E63) FITC Conjugated

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Product code: 10-7529-F

Clone name :	ABM3E63
Clonality :	Monoclonal
Application :	FACS
Reactivity :	Human
Conjugate :	FITC

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Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG1 Kappa
Purification :	Protein G Chromatography
Content :	25 µg in 125 µl/100 µg in 500 µl Tris and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C; stable for 6 months.


Gene :	PECAM1
Gene ID :	5175
Uniprot ID :	P16284
Alternative Name :	PECAM1
Immunogen Information :	Human spleen membranes from a patient with hairy cell leukemia was used as immunogen to generate the CD31 (PECAM-1) antibody.

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules, and plays key roles in leukocyte migration, angiogenesis, and integrin activation. CD31 is expressed on endothelial and hematopoeitic (platelets, monocytes, macrophages, granulocytes, T and B lymphocytes, dendritic, bone marrow stem and adult) cells.

FACS: 0.5µg/10^6 cells

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Cell junction
Post transnational modification:	Palmitoylation by ZDHHC21 is necessary for cell surface expression in endothelial cells and enrichment in membrane rafts.
Tissue Specificity:	Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580308). Expressed on neutrophils (at protein level) (PubMed:17580308). Isoform Long predominates in all tissues examined (PubMed:12433657). Isoform Delta12 is detected only in trachea (PubMed:12433657). Isoform Delta14-15 is only detected in lung (PubMed:12433657). Isoform Delta14 is detected in all tissues examined with the strongest expression in heart (PubMed:12433657). Isoform Delta15 is expressed in brain, testis, ovary, cell surface of platelets, human umbilical vein endothelial cells (HUVECs), Jurkat T-cell leukemia, human erythroleukemia (HEL) and U-937 histiocytic lymphoma cell lines (at protein level) (PubMed:12433657, PubMed:18388311).
BioGrid:	111201. 22 interactions.