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  7. Monoclonal Antibody to EGFR (Epidermal Growth Factor Receptor)(Clone : 31G7)

Monoclonal Antibody to EGFR (Epidermal Growth Factor Receptor)(Clone : 31G7)

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Formalin-fixed, paraffin-embedded human Placenta stained with EGFR Monoclonal Antibody (31G7).

Monoclonal Antibody to EGFR (Epidermal Growth Factor Receptor)(Clone : 31G7)

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Product code: 36-1100

Clone name : 31G7
Clonality : Monoclonal
Application : IHC, FACS, IF
Reactivity : Human

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100 µg
$459.00 

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Download TDS MSDS

  • Product Info
  • Antigen Details
  • Description
  • Application Note
  • Citations (1)
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Format : Purified
Amount : 100 µg
Isotype : Mouse IgG2a Kappa
Purification : Affinity Chromatography
Content : 100 µg in 500 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition : Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.
Gene : EGFR
Gene ID : 1956
Uniprot ID : P00533
Alternative Name : EGFR, ERBB, ERBB1, HER1
Immunogen Information : Human EGFR purified from A431 cells

This MAb recognizes a protein of 170kDa, identified as EGFR. EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2, -3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, beta cellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas.

Flow Cytometry (0.5-1µg/million cells in 0.1ml); Immunofluorescence (1-2µg/ml); Immunohistology (Formalin-fixed) (2-4µg/ml for 30 minutes at RT); (Digest formalin-fixed tissues with Protease at 1mg/ml PBS, pH 7.4 for 10 min at 37C); Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

1. Matsumoto Y, Kobayashi M, Shingu K, et al. An anaplastic pleomorphic xanthoastrocytoma with periventricular extension: An autopsy case report and review of the literature [published online ahead of print, 2020 Jun 23]. Neuropathology. 2020;10.1111/neup.12666. doi:10.1111/neup.12666

Subcellular location: Secreted
Post transnational modification: Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197.
Tissue Specificity: Ubiquitously expressed. Isoform 2 is also expressed in ovarian cancers.
BioGrid: 108276. 1217 interactions.
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Monoclonal Antibody to EGFR (Epidermal Growth Factor Receptor)(Clone : 31G7)

Product code: 36-1100
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