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Fig:1- Cell Surface flow analysis of PD-L1 in 3 day-PHA treated human PBMC cells using 1 µg/10^6 cells of PD-L1 antibody (Clone: ABM5F25). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.
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PD-L1 (CD274/B7-H1) is a critical membrane-bound costimulatory molecule belongs to the B7 superfamily that inhibits immune responses through its receptor, PD-1 and PD-L1 play a key role in the pathogenesis of inflammatory diseases (programmed death 1). It is widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells and regulates inflammatory responses. PD-L1 exerts inflammation regulatory functions via a negative co-stimulatory effect on T cell functions to inhibit cytokine secretion, facilitate apoptosis of activated T cells and induce T cell anergy. Aberrant expression and dysregulation of CD274 have been reported during bacterial infection, inflammation and in numerous autoimmune diseases.
FACS analysis: 0.5-1 µg/10^6 cells; Western blot analysis: 2-4 µg/ml; Immunohistochemical analysis: 5-10 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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