Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)

Fig:1- Cell Surface flow analysis of PD-L1 in 3 day-PHA treated human PBMC cells using 1 µg/10^6 cells of PD-L1 antibody (Clone: ABM5F25). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.

Monoclonal antibody to Human PD-L1 (Clone: ABM5F25)

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Fig:1- Cell Surface flow analysis of PD-L1 in 3 day-PHA treated human PBMC cells using 1 µg/10^6 cells of PD-L1 antibody (Clone: ABM5F25). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.

Fig-2: Cell surface flow analysis of PD-L1 in CHO-PD-L1 transfected cell line using 0.5 µg/10^6 cells of PD-L1 antibody (Clone: ABM5F25). Green represents isotype control; red represents anti-PD-L1 antibody. Goat anti-mouse PE conjugate was used as secondary antibody.

Fig-3: Western blot analysis of PDL1. Anti-PD-L1 antibody (Clone: ABM5F25) was tested at 0.5 µg/ml on Recombinant lysate.

Fig-4: Western blot analysis of PDL1. Anti-PD-L1 antibody (Clone: ABM5F25) was tested at 2 µg/ml on (1) Daudi and (2) HepG2 lysates.

Fig-5: Immunohistochemical analysis of PD-L1 in Hodkin's Lymphoma tissue using PD-L1 antibody (Clone: ABM5F25) at 5 µg/ml.

Fig-6: Western blot analysis of PDL1. Anti-PD-L1 antibody (Clone: ABM5F25) was tested at 0.5 µg/ml on (1) U87 and (2) THP1 lysates.

Product code: 10-7599

Clone name :	ABM5F25
Clonality :	Monoclonal
Application :	IHC, FACS, WB
Reactivity :	Human

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Format :	Purified
Amount :	100 µg
Isotype :	Mouse IgG2b Kappa
Purification :	Protein G Chromatography
Content :	25 µg in 50 µl/100 µg in 200 µl PBS containing 0.05% BSA and 0.05% sodium azide. Sodium azide is highly toxic.
Storage condition :	Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles.


Gene :	CD274
Gene ID :	29126
Uniprot ID :	Q9NZQ7
Alternative Name :	CD274, B7H1, PDCD1L1, PDCD1LG1, PDL1
Immunogen Information :	A partial length recombinant protein of PD-L1 (amino acid 13-224) was used as the immunogen for this antibody.

PD-L1 (CD274/B7-H1) is a critical membrane-bound costimulatory molecule belonging to the B7 superfamily that inhibits immune responses through its receptor, PD-1. PD-L1 plays a key role in the pathogenesis of inflammatory diseases (programmed death 1). It is widely expressed in the mononuclear phagocyte system (MPS), may co-stimulate T cells, and regulates inflammatory responses. PD-L1 exerts inflammation regulatory functions via a negative co-stimulatory effect on T cell functions to inhibit cytokine secretion, facilitates apoptosis of activated T cells, and induces T cell anergy. Aberrant expression and dysregulation of CD274 have been reported during bacterial infection, inflammation, and in numerous autoimmune diseases.

FACS analysis: 0.5-1 µg/10^6 cells; Western blot analysis: 2-4 µg/ml; Immunohistochemical analysis: 5-10 µg/ml

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location:	Endomembrane system
Post transnational modification:	Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.
Tissue Specificity:	Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
BioGrid:	118891. 48 interactions.