NALE™ Monoclonal Antibody to TLR9 (Clone: ABM1C51)
|Format :||Azide Free,Purified|
|Amount :||100 µg|
|Isotype :||Mouse IgG1 Kappa|
|Purification :||Protein G Chromatography|
|Content :||25 µg in 50 µl/100 µg in 200 µl PBS containing No Azide, low endotoxin (0.1EU/1ug).|
|Storage condition :||Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.|
TLR9, a member of toll-like receptor family are central to the innate immunity by identifying pathogen associated molecular patterns (PAMPs). TLR9 identify unmethylated CpG dinucleotides present in bacterial DNA leading to NF-kB activation.
Western blot analysis: 2-4 µg/ml, FACS analysis: 0.5 µg/10^6 cells, Immunohistochemical analysis: 5 µg/ml
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Subcellular location:||Endoplasmic reticulum membrane, Endosome, Lysosome, Cytoplasmic vesicle|
|Post transnational modification:||Activated by proteolytic cleavage of the flexible loop between repeats LRR14 and LRR15 within the ectodomain. Cleavage requires UNC93B1. Proteolytically processed by first removing the majority of the ectodomain by either asparagine endopeptidase (AEP) or a cathepsin followed by a trimming event that is solely cathepsin mediated and required for optimal receptor signaling.|
|Tissue Specificity:||Highly expressed in spleen, lymph node, tonsil and peripheral blood leukocytes, especially in plasmacytoid pre-dendritic cells. Levels are much lower in monocytes and CD11c+ immature dendritic cells. Also detected in lung and liver.|
|BioGrid:||119902. 20 interactions.|