NFAT Leeporter™ Luciferase Reporter-Jurkat Cell Line
Fig-1: Induction of NFAT activity by anti-CD3 antibody in the presence or absence of anti-CD28 antibody at 10 ug/ml in NFAT Leeporter™ - Jurkat T cells.
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Shipping Info:
Order now and get it on Thursday December 12, 2024
Same day delivery FREE on San Diego area orders placed by 1.00 PM
Amount : | 1 Vial |
Content : | Each vial contains 2 ~ 3 x 10^6 cells in 1 ml of 90% FBS + 10% DMSO. |
Storage condition : | Immediately upon receipt, store in liquid nitrogen. |
The NFAT Leeporter™ Luciferase Reporter cell line is a stably transfected Jurkat T cell line which expresses Renilla luciferase reporter gene under the transcriptional control of the Nuclear Factor of Activated T-cells (NFAT) response element, so that the cell line is designed to measure the transcriptional activity of NFAT. NFAT is a transcription factor originally found in activated T lymphocytes, and is now known to regulate not only T cell activation and differentiation but also the function of other immune cells including dendritic cells, B cells and megakaryocytes. The NFAT induction by anti-CD3 antibody and ionomycin is shown in Figures 1 through 4.
Application:
- Monitor the NFAT signaling pathway activity.
- Screen for activators or inhibitors of the NFAT signaling pathway.
Culture conditions:
To passage the cells, transfer cells to a tube, spin down cells, resuspend cells and seed appropriate aliquots of cell suspension into new culture vessels. Subcultivation ration = 1:10 to 1:20 weekly. To achieve satisfactory results, cells should not be passaged over 16 times.
Functional validation:
A. Response of NFAT Leeporter™ – Jurkat cells to anti-CD3 antibody.
B. Response of NFAT Leeporter™ – Jurkat cells to Ionomycin.
LIMITED USE RESTRICTIONS:
THIS PRODUCT IS SOLELY FOR IN VITRO RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC USE.
By use of this product, user agrees to be bound by the terms of this limited use statement.
This product is solely for Internal Research Purposes and not for Commercial Purposes. Commercial Purposes include, but are not limited to (1) use of the cell line in manufacturing; (2) use of the cell line to provide a service, information or data; (3) use of the cell line for therapeutic, diagnostic or prophylactic purposes; or (4) resale of the cell line whether or not such cell lines are resold for use in research. The buyer cannot sell, give or otherwise transfer this product to a third party.
Commercial License Agreement is available for non-research use if applicable. Please contact Abeomics ([email protected]).
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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