|Alternative Name :||Calcitonin, Preprocalcitonin, Calca.|
|Amount :||10 µg|
Filtered White lyophilized (freeze-dried) powder.
Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, an N terminal residue, calcitonin and katacalcin. Levels of unprocessed procalcitonin rise significantly after bacterial infection, trauma or shock.
Procalcitonin Porcine Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain (Thr26-Asn141) containing 126 amino acids including a 10 aa His tag at N-terminus. The total calculated molecular mass is 14.1kDa.
|Purification :||Greater than 95.0% as determined by SDS-PAGE.|
|Content :||Procalcitonin was filtered (0.4µm) and lyophilized in 20mM TRIS and 50mM NaCl, pH 8.0.
It is recommended to add deionized water to prepare a working stock solution of approximately 0.5 mg/ml and let the lyophilized pellet dissolve completely. Procalcitonin is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.
|Storage condition :||Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.|
|Amino acid sequence :||MKHHHHHHASTPLRSALETL PDPGPLSEKE GRLLLAALVK AYVQRKTNEL EQEQEQETEG SSLDSSRAKR CSNLSTCVLS AYWRNLNNFH RFSGMGFGPE TPGKKSDIAS SLERDLFPRG MPQDAN.|