Rat IgG2a Isotype Control Antibody (Clone : S193)
Figure 1 : Flow cytometry surface nonspecific staining pattern of human peripheral whole blood stained using rat IgG2a Isotype control (RTG2A1-1) purified antibody (concentration in sample 3 µg/ml, DAR APC).
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| Format : | Purified |
| Amount : | 0.1 mg |
| Isotype : | Rat IgG2a |
| Purification : | Purified by protein-A affinity chromatography |
| Content : | 1 mg/ml Formulation : Phosphate buffered saline (PBS) solution with 15 mM sodium azide |
| Storage condition : | Store at 2-8°C. Do not freeze. |
Negative control: The reagent is intended as an isotype control to establish the amount of non-specific antibody binding. For your particular experiment, use the same concentration of this control antibody as the recommended working concentration of the antigen-specific antibody. Also, when working with prediluted antibodies, dilute the isotype control to the same concentration as is the concentration of the antigen-specific antibody in the prediluted antibody solution you are using. If under particular experimental conditions the background signal of the isotype control is too high (usually when working concentrations of used antibodies are above 10 µg/ml of incubation mixture), change the conditions of your experiment to reduce the background.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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