Recombinant Anti-SARS-CoV-2 Spike RBD antibody (ABMX-002)
Fig-1: Neutralization of binding between SARS-Cov-2 Spike RBD protein and ACE2 receptor protein by the Recombinant Anti-SARS-CoV-2 Spike RBD antibody (ABMX-002). An ELISA plate coated with human ACE2 protein (Abeomics, Cat. No. 32-190010) was incubated with various concentrations of RBD antibody (ABMX-002) in the presence of biotinylated SARS-Cov-2 Spike RBD protein (Abeomics, Cat. No. 21-1005-B) and analyzed using HRP-Streptavidin for detection.
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|Amount :||100 µg|
|Isotype :||Human IgG1 kappa|
|Purification :||Affinity purified|
|Content :||0.5 mg/ml, 100 ug in sterile PBS|
|Storage condition :||Store the antibody at 4°C, stable for 6 months. For long-term storage, store at -20°C. Avoid repeated freeze and thaw cycles.|
|Uniprot ID :||P0DTC2|
The spike (S) glycoprotein of coronaviruses contains protrusions that will only bind to certain receptors on the host cell. Known receptors bind S1 are ACE2, angiotensin-converting enzyme 2; DPP4, dipeptidyl peptidase-4; APN, aminopeptidase N; CEACAM, carcinoembryonic antigen-related cell adhesion molecule 1; Sia, sialic acid; O-ac Sia, O-acetylated sialic acid. The spike is essential for both host specificity and viral infectivity. The term 'peplomer' is typically used to refer to a grouping of heterologous proteins on the virus surface that function together. The spike (S) glycoprotein of coronaviruses is known to be essential in the binding of the virus to the host cell at the advent of the infection process. It's been reported that 2019-nCoV can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity. The main functions for the Spike protein are summarized as: Mediate receptor binding and membrane fusion; Defines the range of the hosts and specificity of the virus; Main component to bind with the neutralizing antibody; Key target for vaccine design; Can be transmitted between different hosts through gene recombination or mutation of the receptor binding domain (RBD), leading to a higher mortality rate.
1. Archana Thomas, William B Messer, Donna E Hansel, Daniel N Streblow, Steven C Kazmierczak, Zoe L Lyski, Zhengchun Lu, Mark K Slifka, Establishment of Monoclonal Antibody Standards for Quantitative Serological Diagnosis of SARS-CoV-2 in Low Incidence Settings, Open Forum Infectious Diseases, 2021;, ofab061, https://doi.org/10.1093/ofid/ofab061