Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag

Figure-1: ELISA detection of Anti-SARS-CoV-2 Nucleocapsid antibody (ABMX-004) cat. no. 10-2007. An ELISA plate coated with 100 ng of Nucleocapsid protein (Abeomics, Cat. No. 21-1003) was incubated over night at 4C. Different concentrations of Anti-Nucleocapsid recombinant human antibody (ABMX-004) was added to the plate. Goat anti-human Fc HRP was used as detection antibody.

Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag

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Figure-1: ELISA detection of Anti-SARS-CoV-2 Nucleocapsid antibody (ABMX-004) cat. no. 10-2007. An ELISA plate coated with 100 ng of Nucleocapsid protein (Abeomics, Cat. No. 21-1003) was incubated over night at 4C. Different concentrations of Anti-Nucleocapsid recombinant human antibody (ABMX-004) was added to the plate. Goat anti-human Fc HRP was used as detection antibody.

Figure-2: Wells of a 96-microtiter plate were coated with 4µg/ml of Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag. The binding was detected by addition of different dilution of Coronavirus (COVID-19) Nucleocapsid Antibody (Abeomics Cat. No. 11-2003). The reactivity was detected by a HRP-conjugated goat-anti-rabbit polyclonal antibody.

Figure-3: SDS-PAGE analysis of purified Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag. 2 µg protein was run on a 4-20% SDS-PAGE gel followed by Coomassie blue staining.

Figure-4: The checkerboard Sandwich ELISA was carried out by coating the 96 well plate with 200 ng of ABM4H11.1C12 (Cat#10-10035) monoclonal antibody. The nucleocapsid protein was serially diluted from 10 ng to 0.0004ng in triplicates across rows. The 11-2004 detection antibody was applied in serial dilution starting with 200ng/ well across column

Product code: 21-1003

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1 mg
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100 μg

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Amount :	100 μg
Purification :	>95% by SDS-PAGE.
Content :	0.5 mg/ml in 50 mM Tris, pH-7.4, 300 mM NaCl and 10% Glycerol
Storage condition :	Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag is shipped on ice packs. Upon arrival, Store at -20°C. Do not freeze-thaw multiple times.
AA sequence :	The target protein is expressed with sequence (Ser2-Ala419) of SARS-CoV-2/COVID-19 Nucleocapsid fused with an 6×His tag in N-Terminal.


Gene :	N
Uniprot ID :	P0DTC9
Alternative Name :	Nucleocapsid protein;NP;Nucleoprotein;cov np;Protein N

Source: E. coli. Recombinant SARS-CoV-2/COVID-19 Nucleocapsid Protein His Tag is produced by E. coli expression system. The target protein is expressed with sequence (Ser2-Ala419) of SARS-CoV-2/COVID-19 Nucleocapsid fused with an 6×His tag in N-Terminal.