Recombinant SARS-CoV-2 Spike S1 Protein with His tag and Avi
Figure-1: Recombinant 2019-nCoV Spike S1 protein on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
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| Amount : | 100 μg |
| Purification : | >95% by SDS-PAGE. |
| Content : | Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. |
| Storage condition : | Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week. Avoid repeated freeze/thaw cycles. |
| AA sequence : | The target protein is expressed with sequence (Gln14-Arg683) of 2019-ncov Spike S1 fused with a 6×His tag and Avi at the C-terminus. |
| Gene ID : | 43740568 |
| Alternative Name : | S1 protein; Spike glycoprotein Subunit1; S glycoprotein Subunit1; Spike protein S1; novel coronavirus s1 Protein |
Source: HEK 293 cells. The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates this interaction.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Bio-Activity: Measured by its binding ability in a functional ELISA. Immobilized Recombinant 2019-nCoV Spike S1-His at 2μg/mL (100 μL/well) can bind Recombinant Human ACE2 with a linear range of 1.5-15 ng/mL. Endotoxin : < 1.0 EU/μg of the protein by LAL method.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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