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  7. Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)

Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)

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Fig. 1: Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).

Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)
Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)
Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)
Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)
Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)

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Fig. 1: Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
Fig. 2: Immunofluorescence staining of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG conjµgated to CF488 (green). Membrane stained with Phalloidin (Red).
Fig. 3: Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with B7-H4 Mouse Monoclonal Antibody (B7H4/1788).
Fig. 4: SDS-PAGE Analysis Purified B7-H4 Mouse Monoclonal Antibody (B7H4/1788).
Fig. 5: Western Blot of HCT116 cell lysates using B7-H4 Mouse Monoclonal Antibody (B7H4/1788).

Product code: 36-3399

Clone name : B7H4/1788
Clonality : Monoclonal
Application : ELISA, FACS, IF, WB, IHC
Reactivity : Human

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  •   20 µg

  •  100 µg

  • $199.00 

  • $459.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Mouse IgG2a, kappa
Content : 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Storage condition : Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous.
Gene : VTCN1
Gene ID : 79679
Uniprot ID : Q7Z7D3
Alternative Name : B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7S1; Immune costimulatory protein B7-H4; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VCTN1
Immunogen Information : A recombinant fragment of human B7-H4 protein (exact sequence is proprietary)
T cell activation and immune function are regulated by the innate immune system throµgh positive and negative costimulatory proteins. One such protein, B7-H4 (B7-homolog 4), belongs to the B7 immunoglobulin superfamily of ligand-lymphocyte interacting proteins. Expressed primarily on the membrane of lymphoid cells, B7-H4 is an immuno-inhibitory protein that interacts with receptors on the surface of T lymphocytes, thus mediating cellular and humoral immune responses. Overexpression of the B7-H4 protein is associated with certain malignancies, including ovarian and breast cancer, as its interaction with T cells suppresses tumor-associated immunity. Current research sµggests that, similar to Mucin 16 (CA-125), B7-H4 may be a useful biomarker for the early detection of ovarian cancer.

ELISA (Use Ab at 2-4µg/ml for coating) (Order Ab without BSA); Flow Cytometry (1-2µg/million cells in 0.1ml); ,Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-B7-H4 (Immuno-Inhibitory Protein) Monoclonal Antibody(Clone: B7H4/1788)

Product code: 36-3399
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