Fig. 1: Flow Cytometric Analysis of SKBR-3 cells using B7-H4 Mouse Monoclonal Antibody (B7H4/1788) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).
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Product code: 36-3399
| Clone name : | B7H4/1788 |
| Application : | ELISA, FACS, IF, WB, IHC |
| Reactivity : | Human |
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| Gene : | VTCN1 |
| Gene ID : | 79679 |
| Uniprot ID : | Q7Z7D3 |
| Alternative Name : | B7 family member, H4; B7 homolog 4; B7 superfamily member 1; B7-H4; B7S1; Immune costimulatory protein B7-H4; T-cell costimulatory molecule B7x; V-set domain-containing T-cell activation inhibitor 1; VCTN1 |
| Amount : | 100 µg |
| Isotype : | Mouse IgG2a, kappa |
| Immunogen Information : | A recombinant fragment of human B7-H4 protein (exact sequence is proprietary) |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
ELISA (Use Ab at 2-4µg/ml for coating) (Order Ab without BSA); Flow Cytometry (1-2µg/million cells in 0.1ml); ,Immunofluorescence (1-2µg/ml); Western Blot (1-2µg/ml);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
