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  7. Anti-CD14 / LPS-receptor Monoclonal Antibody (Clone:MEM-18)

Anti-CD14 / LPS-receptor Monoclonal Antibody (Clone:MEM-18)

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Anti-CD14 / LPS-receptor Monoclonal Antibody (Clone:MEM-18)

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Product code: 30-1118

Clone name : MEM-18
Clonality : Monoclonal
Application : FACS
Reactivity : Non-Human Primates

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0.1 mg
$339.00 

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : CD14
Gene ID : 929
Uniprot ID : P08571
Alternative Name : CD14
Immunogen Information : A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.

The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. In human, the epitope recognized by MEM-18 is located between amino acids 57-64.

Flow Cytometry Recommended dilution:4 µg/ml
Immunoprecipitation Western Blotting ELISA The antibody MEM-18 has been tested as the detection antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody B-A8 . Functional Application The antibody MEM-18 completely blocks binding of fluorescein (FITC) - labeled bacterial LPS to the monocyte surface and it also blocks the binding of CD14 to the extracellular TLR2 domain.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cell membrane, Secreted, Membrane raft, Golgi apparatus
Post transnational modification: N- and O- glycosylated. O-glycosylated with a core 1 or possibly core 8 glycan.
Tissue Specificity: Detected on macrophages (at protein level) (PubMed:1698311). Expressed strongly on the surface of monocytes and weakly on the surface of granulocytes; also expressed by most tissue macrophages.
BioGrid: 107367. 35 interactions.
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Anti-CD14 / LPS-receptor Monoclonal Antibody (Clone:MEM-18)

Product code: 30-1118
*specify in mg or ml

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