Anti-CD14 (Monocyte / Macrophage Marker) Monoclonal Antibody(Clone: LPSR/2385)
Fig. 1: Formalin-fixed, paraffin-embedded human Lymph Node stained with CD14-Monospecific Mouse Monoclonal Antibody (LPSR/2385).
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| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | CD14 |
| Gene ID : | 929 |
| Uniprot ID : | P08571 |
| Alternative Name : | Lipopolysaccharide receptor; LPS-R; LPSR; Mo2; Monocyte Differentiation Antigen 14; Myeloid cell-specific leucine-rich glycoprotein |
| Immunogen Information : | Recombinant fragment of human CD14 protein (around aa 25-148) (exact sequence is proprietary) |
Recognizes a protein of 55kDa, identified as CD14 (also known lipopolysaccharide receptor). CD14 is expressed strongly on monocytes and macrophage and weakly on the surface of neutrophils. CD14 is anchored to cells by linkage to glycosylphosphatidylinositol (GPI) and functions as a high affinity receptor for complexes of LPS and LPS binding protein (LBP). Soluble CD14, also binding to LPS, acts at physiological concentration as an LPS agonist and has, at higher concentrations, an LPS antagonizing effect in cell activation.
Western Blot (1-2µg/ml); Flow Cytometry (1-2µg/million cells);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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