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  7. Anti-CD27 antibody(DM58), Rabbit mAb

Anti-CD27 antibody(DM58), Rabbit mAb

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Figure 1. Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1µg/ml (clone: DM58) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.

Anti-CD27 antibody(DM58), Rabbit mAb
Anti-CD27 antibody(DM58), Rabbit mAb
Anti-CD27 antibody(DM58), Rabbit mAb

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Figure 1. Expi 293 cell line transfected with irrelevant protein (A) and human CD27 (B) were surface stained with Rabbit anti-CD27 monoclonal antibody 1µg/ml (clone: DM58) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. FACS data of serially titrated Rabbit anti-CD27 monoclonal antibody (clone: DM58) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 3. Affinity ranking of different Rabbit anti-CD27 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Product code: 12-9045

Clone name : DM58
Clonality : Monoclonal
Application : ELISA, FACS
Reactivity : Human

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100 µg
$599.00 

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Amount : 100 µg
Isotype : Rabbit IgG
Purification : Purified from cell culture supernatant by affinity chromatography
Content : Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Storage condition : Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Alternative Name : CD27, TNFRSF7, S152, T14, Tp55
Immunogen Information : Recombinant human CD27 (Ala20-Arg191) produced by using human HEK293 cells

The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is required for generation and long-term maintenance of T cell immunity. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis. This receptor transduces signals that lead to the activation of NF-kappaB and MAPK8/JNK. Adaptor proteins TRAF2 and TRAF5 have been shown to mediate the signaling process of this receptor. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor.

Recommended Dilutions ELISA 1/5000-10000;FACS  1/100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-CD27 antibody(DM58), Rabbit mAb

Product code: 12-9045
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