Fig. 1: Formalin-fixed, paraffin-embedded human Tonsil stained with CD27 Mouse Monoclonal Antibody (LPFS2/1611).
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Product code: 36-3555
| Clone name : | LPFS2/1611 |
| Application : | ELISA, FACS, IF, IHC |
| Reactivity : | Human |
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| Gene : | CD27 |
| Gene ID : | 939 |
| Uniprot ID : | P26842 |
| Alternative Name : | LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7 |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Immunogen Information : | Recombinant full-length human CD27 protein |
Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
ELISA (For coating, order antibody without BSA);Flow Cytometry (1-2µg/million cells); Immunofluorescence (1-2µg/ml);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
