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  7. Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:4H11[APG])

Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:4H11[APG])

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Figure-1: Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using anti-CD34 (4H11[APG]) purified / GAM-APC.

Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:4H11[APG])
Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:4H11[APG])

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Figure-1: Flow cytometry surface staining pattern of human peripheral whole blood showing CD34 positive stem cells (red) stained using anti-CD34 (4H11[APG]) purified / GAM-APC.
Figure-2: Western bloting analysis of human CD34 using mouse monoclonal antibody 4H11[APG] on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody 4H11[APG] followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.

Product code: 30-1185

Clone name : 4H11[APG]
Clonality : Monoclonal
Reactivity : Human

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0.1 mg
$339.00 

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Format : Purified
Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified by precipitation and chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : CD34
Gene ID : 947
Uniprot ID : P28906
Alternative Name : CD34
Immunogen Information : Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.

CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Western blotting: Recommended dilution: 1-2 μg/ml; positive control: Kg-1a, TF-1 cells, non-reducing conditions.
Flow cytometry: Recommended dilution: 2 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: placenta endothelium.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Membrane
Post transnational modification: Phosphorylated on serine residues by PKC.
Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
BioGrid: 107385. 1 interactions.
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Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:4H11[APG])

Product code: 30-1185
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