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  7. Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free

Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free

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Figure-1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).

Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free
Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free
Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free
Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free
Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free

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Figure-1: Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).
Figure-2: Flow cytometry multicolor surface staining of human peripheral blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0,6 μg/ml, GAM APC, red-filled) and anti-human CD45 (MEM-28) Pacific Blue™ antibody (10 μl reagent / 100 μl of peripheral whole blood).
Figure-3: Separation of human CD45dim CD34 positive stem cells (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD34 (QBEnd-10) purified antibody (concentration in sample 0,6 μg/ml, GAM APC).
Figure-4: Western blotting analysis of human CD34 using mouse monoclonal antibody QBEnd-10 on lysates of TF-1 cell line and HEK293T/17 cell line (CD34 non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD34 monoclonal antibody QBEnd-10 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for CD34 protein at approximately 110 kDa.
Figure-5: Flow cytometry analysis (surface staining) of CD34 in human peripheral blood with anti-CD34 (QBEnd-10) azide free.

Product code: 30-1028

Clone name : QBEnd-10
Clonality : Monoclonal
Application : FACS
Reactivity : Human

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$339.00 

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Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : CD34
Gene ID : 947
Uniprot ID : P28906
Alternative Name : CD34
Immunogen Information : Human endothelial vesicles

CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
Flow cytometry: Recommended dilution: 5 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml, positive control: TF-1 cells.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Membrane
Post transnational modification: Phosphorylated on serine residues by PKC.
Tissue Specificity: Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.
BioGrid: 107385. 1 interactions.
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Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free

Product code: 30-1028
*specify in mg or ml

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