Anti-CD34 / Mucosialin Monoclonal Antibody (Clone:QBEnd-10)-Azide free
|Amount :||0.1 mg|
|Isotype :||Mouse IgG1|
|Purification :||Purified by protein-A affinity chromatography|
|Storage condition :||Store at 2-8°C. Do not freeze.|
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Functional application: The antibody QBEnd-10 induces homotypic adhesion of leukemic cell line.
Flow cytometry: Recommended dilution: 5 μg/ml.
Immunohistochemistry (paraffin sections): Recommended dilution: 2-8 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml, positive control: TF-1 cells.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
|Post transnational modification:||Phosphorylated on serine residues by PKC.|
|Tissue Specificity:||Selectively expressed on hematopoietic progenitor cells and the small vessel endothelium of a variety of tissues.|
|BioGrid:||107385. 1 interactions.|