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  7. Anti-CD28 antibody(DM63), Rabbit mAb

Anti-CD28 antibody(DM63), Rabbit mAb

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Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD28 protein, mFc-His tagged protein can bind Rabbit anti-CD28 monoclonal antibody (clone: DM63) in a linear range of 1-100 ng/ml.

Anti-CD28 antibody(DM63), Rabbit mAb
Anti-CD28 antibody(DM63), Rabbit mAb
Anti-CD28 antibody(DM63), Rabbit mAb
Anti-CD28 antibody(DM63), Rabbit mAb

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Figure 1. ELISA plate pre-coated by 2 µg/ml (100 µl/well) Human CD28 protein, mFc-His tagged protein  can bind Rabbit anti-CD28 monoclonal antibody (clone: DM63) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CD28 (B) were surface stained with Rabbit anti-CD28 monoclonal antibody 1µg/ml (clone: DM63) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. FACS data of serially titrated Rabbit anti-CD28 monoclonal antibody (clone: DM63) on Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti- CD28 mAb clones by titration of different concentration onto Jurkat cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.

Product code: 12-9050

Clone name : DM63
Clonality : Monoclonal
Application : ELISA, FACS
Reactivity : Human

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100 µg
$599.00 

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  • Product Info
  • Antigen Details
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Amount : 100 µg
Isotype : Rabbit IgG
Purification : Purified from cell culture supernatant by affinity chromatography
Content : Preservative: 0.1% Procline 300
Constituents: 50% Glycerol; PBS,pH 7.4; 0.1% BSA
Storage condition : Store at -20°C for 12 months (Avoid repeated freezing and thawing)
Alternative Name : CD28, Tp44
Immunogen Information : Recombinant human CD28 (Asn19-Pro152) produced by using human HEK293 cells

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Recommended Dilutions ELISA 1/5000-10000;FACS  1/100

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

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Anti-CD28 antibody(DM63), Rabbit mAb

Product code: 12-9050
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