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  7. Anti-CD81 Monoclonal Antibody (Clone:M38)-Low Endotoxin

Anti-CD81 Monoclonal Antibody (Clone:M38)-Low Endotoxin

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Figure-1: Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Anti-CD81 Monoclonal Antibody (Clone:M38)-Low Endotoxin
Anti-CD81 Monoclonal Antibody (Clone:M38)-Low Endotoxin
Anti-CD81 Monoclonal Antibody (Clone:M38)-Low Endotoxin

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Figure-1: Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.
Figure-2: Western blotting analysis of human CD81 using mouse monoclonal antibody M38 on lysate of Jurkat cell line under non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-human CD81 monoclonal antibody M38 followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD81 at approximately 25 kDa.
Figure-3: Separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD81 (M38) purified antibody (low endotoxin, concentration in sample 4 μg/ml) GAM APC.

Product code: 30-1618

Clone name : M38
Clonality : Monoclonal
Application : FACS
Reactivity : Human

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0.1 mg
$356.00 

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Format : Low Endotoxin
Amount : 0.1 mg
Isotype : Mouse IgG1
Purification : Purified by protein-A affinity chromatography
Storage condition : Store at 2-8°C. Do not freeze.
Gene : CD81
Gene ID : 975
Uniprot ID : P60033
Alternative Name : CD81, TAPA1, TSPAN28
Immunogen Information : MOLT-4 (human T-ALL cell line)

CD81 (TAPA-1), a member of the tetraspanin family, is expressed on virtually all nucleated cells, but above all on germinal center B cells. CD81 forms complexes with other tetraspanin proteins, integrins, coreceptors, MHC class I and II molecules, and influences adhesion, morphology, activation, proliferation and differentiation of B, T and other cells - e.g. in muscles CD81 promotes cell fusion and myotube maintenance. CD81 has been also identified as a receptor for the hepatitis C virus.

Functional application: In human MOLT-4 T-cell line the antibody M38 inhibits syncytium formation induced by coculture with human T-cell leukemia virus type 1 (HTLV-1)-positive human T-cell lines.
Flow cytometry: recommended dilution: 1 μg/ml.
Western blotting: recommended dilution: 1-2 μg/ml; positive control: Jurkat cells, non-reducing conditions.

 

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Basolateral cell membrane
Post transnational modification: Not glycosylated.
Tissue Specificity: Hematolymphoid, neuroectodermal and mesenchymal tumor cell lines.
BioGrid: 107413. 201 interactions.
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Anti-CD81 Monoclonal Antibody (Clone:M38)-Low Endotoxin

Product code: 30-1618
*specify in mg or ml

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