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  7. Biotin Conjugated, Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DA-7)

Biotin Conjugated, Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DA-7)

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Figure-1: Western blotting analysis of human cytokeratin 18 using mouse monoclonal antibody DA-7 on lysates of HT-29 cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of biotinylated mouse anti-cytokeratin 18 monoclonal antibody followed by IRDye800-conjugated streptavidin. Cytokeratin 18 was detected at approximately 46 kDa.

Biotin Conjugated, Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DA-7)

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Product code: 30-1829

Clone name : DA-7
Clonality : Monoclonal
Application : IP, WB, IHC, ICC, ELISA
Reactivity : Human
Conjugate : Biotin

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0.1 mg
$383.00 

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Amount : 0.1 mg
Isotype : Mouse IgG1
Storage condition : Store at 2-8°C. Do not freeze.
Gene : KRT18
Gene ID : 3875
Uniprot ID : P05783
Alternative Name : KRT18, CYK18, PIG46
Immunogen Information : Human breast carcinoma cell line PMC-42.

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.Cytokeratins 18 belongs to type I family (acidic cytokeratins).

Western blotting: Recommended dilution: 1-2 μg/ml.

For Research Use Only. Not for use in diagnostic/therapeutics procedures.

Subcellular location: Cytoplasm, Nucleus
Post transnational modification: O-GlcNAcylation increases solubility, and decreases stability by inducing proteasomal degradation.
Tissue Specificity: Expressed in colon, placenta, liver and very weakly in exocervix. Increased expression observed in lymph nodes of breast carcinoma.
BioGrid: 110073. 113 interactions.
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Biotin Conjugated, Anti-Cytokeratin 18 Monoclonal Antibody (Clone:DA-7)

Product code: 30-1829
*specify in mg or ml

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