Anti-Cytokeratin 20 (KRT20) (Colorectal Epithelial Marker) Monoclonal Antibody(Clone: SPM140)
Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Cytokeratin 20 (KRT20) Mouse Monoclonal Antibody (SPM140).
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| Amount : | 100 µg |
| Isotype : | Mouse IgG2b, kappa |
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
| Gene : | KRT20 |
| Gene ID : | 54474 |
| Uniprot ID : | P35900 |
| Alternative Name : | CK20; Cytokeratin-20; K20; KA20; Keratin 20; keratin 20, type I; Keratin type I cytoskeletal 20; Keratin-20; KRT20 |
| Immunogen Information : | Recombinant fragment of human KRT20 protein (around aa 196-323) (exact sequence is proprietary) |
This MAb recognizes an intermediate filament protein of 46kDa, identified as cytokeratin 20 (KRT20). KRT is abundantly expressed in goblet cells and enterocytes of the gastrointestinal tract. It is a useful marker of pancreatic and colorectal cancer. KRT20 is expressed under normal, hyperplastic and neoplastic conditions.It has been detected in adenocarcinomas of the colon, stomachand biliary tract. Breast carcinomas are generally non-reactive.
Western Blot (0.5-2µg/ml);Immunohistochemistry (Formalin-fixed) (1-2µg/ml for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
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