Fig. 1: SDS-PAGE Analysis Purified Cytokeratin 5/8 Monoclonal Antibody (C-50). Confirmation of Purity and Integrity of Antibody.
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Product code: 36-3733
| Clone name : | C-50 |
| Application : | FACS, IF, WB, IHC |
| Reactivity : | Human, Pig, Mouse, Rat, Bovin, Dog, Sheep |
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| Gene : | KRT5 & KRT8 |
| Gene ID : | 3852; 3856 |
| Uniprot ID : | P13647 ; P05787 |
| Alternative Name : | CK5; DDD; EBS2; Epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne Types; K5; Keratin complex 2, basic, Gene 5; Keratin Type II Cytoskeletal 5; Keratin-5; KRT5; KRT5A; Type II Cytoskeletal 5; Type-II Keratin Kb5. CK8; CARD2; CYK8; CYKER; Cytokeratin Endo A; DreK8; EndoA; K2C8; K8; Keratin 8; Krt 2.8; KRT8; Type-II Keratin Kb8 |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Immunogen Information : | Cytoskeletal preparation from HeLa cells |
It reacts with keratin 5 (58kDa) and keratin 8 (52.5kDa). Simple epithelia express cytokeratin 8 in combination with 18. On the other hand, basal cells of stratified epithelia express cytokeratin 5 paired with 14. This antibody therefore, reacts with a wide range of epithelia and their carcinomas.
| Content : | 0.5 ml at 200 µg/ml of antibody Purified from Bioreactor Concentrate by Protein A/G chromatography. Supplied in 10mM PBS, pH 7.4 with 0.05% BSA and 0.05% sodium azide. Contact us if you require this antibody in a different format. |
| Storage condition : | Store at 2 to 8°C. Antibody is stable for 24 months. |
Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (0.5-1.0µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT)(For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 15 min at RT)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
