Fig. 1: Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Multi Cytokeratin Mouse Monoclonal Antibody (C11).
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Product code: 36-3722
| Clone name : | C11 |
| Application : | FACS, IF, WB, IHC |
| Reactivity : | Human, Bovin, Rat, Mouse, Guinea Pig, Frog, Goat, Pig |
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| Gene : | KRT4; KRT5; KRT6; KRT8; KRT10; KRT13; KRT18 |
| Gene ID : | 3851; 3852; 3853; 3856; 3858; 3860; 3875 |
| Uniprot ID : | P19013; P13647; P02538 ; P05787; P13645; P13646; P05783 |
| Amount : | 100 µg |
| Isotype : | Mouse IgG1, kappa |
| Immunogen Information : | Keratin-enriched preparation from cultured human A431 |
This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
| Content : | 200 µg/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml. |
| Storage condition : | Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. |
Flow Cytometry (0.5-1µg/million cells); Immunofluorescence (1-4µg/ml); Western Blot (0.5-1.0µg/ml); Immunohistochemistry (Formalin-fixed) (0.5-1.0µg/ml for 30 min at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
